Accumulation and storage of Zn2+ by Candida utilis

Journal of General Microbiology
M L FaillaE D Weinberg


Starved cells of Candida utilis accumulated Zn2+ by two different processes. The first was a rapid, energy- and temperature-independent system that probably represented binding to the cell surface. The cells also possessed an energy-, pH-, and temperature-dependent system that was capable of accumulating much greater quantities of the cation than the binding process. The energy-dependent system was inhibited by KCN, Na2HAsO4, m-chlorophenyl carbonylcyanide hydrazone, N-ethylmaleimide, EDTA and diethylenetriaminepenta-acetic acid. The system was specific inasmuch as Ca2+, Cr3+, Mn2+, Co2+ or Cu2+ did not compete with, inhibit, or enhance the process, Zn2+ uptake was inhibited by Cd2+. The system exhibited saturation kinetics with a half-saturation value of 1.3 muM and a maximum rate of 0.21 (nmol Zn2+) min(-1) (mg dry wt(-1)) at 30 degrees C. Zn2+ uptake required intact membranes since only the binding process was observed in the presence of nystatin, toluene, or sodium dodecyl sulphate. Cells did not exchange recently accumulated toluene, or sodium dodecyl sulphate. Cells did not exchange recently accumulated 65Zn following the addition of a large excess of non-radioactive Zn2+. Similarly, cells pre-loaded with 65Zn did not los...Continue Reading


Mar 1, 1986·Bulletin of Environmental Contamination and Toxicology·J W Bhattacherjee
Dec 1, 1982·Microbial Ecology·W R BlairW P Iverson
Dec 1, 1977·Microbial Ecology·G M Gadd, A J Griffiths
Dec 1, 1988·Bulletin of Environmental Contamination and Toxicology·A K Prahalad, G Seenayya
Mar 1, 1996·Applied Microbiology and Biotechnology·P Simmons, I Singleton
Jan 1, 1988·Critical Reviews in Food Science and Nutrition·R NathM Sharma
Dec 23, 2008·Journal of Hazardous Materials·Suntud Sirianuntapiboon, Methinee Boonchupleing
Jan 1, 1993·Environmental Pollution·I BerdicevskyS Yannai
May 30, 2006·Bioresource Technology·Suntud Sirianuntapiboon, Thadchai Hongsrisuwan
May 1, 1988·Biotechnology and Bioengineering·K Akrida-DemertziA A Koutinas
Mar 1, 1984·Biotechnology and Bioengineering·A B Norberg, H Persson
Jan 1, 1986·Biotechnology and Bioengineering·N Friis, P Myers-Keith
Jun 1, 1991·Applied Microbiology and Biotechnology·Ana M MarquésFrancisco Congregado
Jun 1, 1980·European Journal of Biochemistry·M Karin, H R Herschman
Jan 14, 2021·Journal of Fungi·Maria Sousa-SilvaIsabel Soares-Silva

Related Concepts

Candida utilis
Plasma Membrane
Edetic Acid, Sodium Salt
Respiratory Chain
Yeast Proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

Biochimica Et Biophysica Acta
G W Borst-Pauwels
The Journal of Applied Bacteriology
N B OmarJ M Arias
Pflügers Archiv : European journal of physiology
M Batic, P Raspor
© 2021 Meta ULC. All rights reserved