PMID: 4966821Mar 1, 1968

Accumulation in gram-postive and gram-negative bacteria as a mechanism of resistance to erythromycin

Journal of Bacteriology
J C Mao, M Putterman

Abstract

Erythromycin was recovered in high yield after incubation with gram-negative bacteria. The cell-free protein-synthesizing preparation from gram-negative bacteria is equally as susceptible to the antibiotic as is that from gram-positive bacteria. Thus, neither destruction of erythromycin nor the absence of the step susceptible to the antibiotic plays an important role in the resistance mechanism of gram-negative bacteria. A 100-fold difference in accumulation of erythromycin between gram-positive and gram-negative bacteria was observed. This alone explains the resistance of gram-negative bacteria to erythromycin. Furthermore, data showed that the inhibition of growth is closely related to the accumulation of erythromycin. The concentration of intracellular erythromycin in gram-positive bacteria was found to be 44- to 90-fold greater than that of the extracellular medium. However, the antibiotic did not accumulate on the cell walls, nor was the accumulation energy-dependent. It is proposed that it takes place by the binding of erythromycin to the bacterial ribosomes, forming a very stable complex. The dissociation constants of erythromycin-Staphylococcus aureus complex and erythromycin-Bacillus subtilis complex were determined to...Continue Reading

Citations

Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ender VolkanScott J Hultgren
Oct 14, 2016·Applied and Environmental Microbiology·Getahun E AggaTerrance M Arthur
Feb 6, 2016·Materials·Sandra MaginaDmitry Evtuguin
Oct 28, 2016·Critical Reviews in Microbiology·Cláudia GomesJoaquim Ruiz
Apr 12, 2016·Journal of Environmental Quality·Martha R ZwonitzerDouglas R Smith
Sep 24, 2004·The Journal of Biological Chemistry·Martin LovmarMåns Ehrenberg
Jan 24, 2020·Journal of Food Protection·John W SchmidtTerrance M Arthur
Dec 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bee-Ha GanJean-Louis Reymond

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