PMID: 8608159Mar 29, 1996Paper

Accumulation of 1-o-alkyl-2,3-diacylglycerols in cultured rat keratinocytes

Biochimica Et Biophysica Acta
H OkuI Chinen

Abstract

The present study was undertaken to identify the chemical structure of neutral lipid accumulated in cultured rat keratinocytes and to address their metabolism. Neutral lipid of similar mobility with alkyldiacylglycerol was isolated from cultured rat keratinocytes by thin layer chromatography. The long-chain diols derived from the neutral lipids were identified as 1-alkylglycerol based on the mass spectra of their nicotinylidene derivatives. Thus these neutral lipids were identified as 1-o-alkyl-2,3-diacylglycerols (ADAG). Addition of rat serum elevated the level of ADAG with increasing trend of linoleic acid concentration in this fraction. [14C]Acetate added to the confluent plates was incorporated into alkyl- and acyl-chains of ADAG with incubation in 24 h, and remained un-metabolized up to 72 h. This, however, is not the case for the label incorporation into phospholipid and triacylglycerol. Radioactivities of these two lipid fractions appeared to reach the maximum in 24 h, and thereafter decreased to 72 h with a similar decay curve. Incorporation of [14C]acetate into phospholipid and ADAG was significantly depressed, and that into triacylglycerol and free cholesterol was increased by the supplementation of the medium with ra...Continue Reading

References

Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J BrodM Ponec
Jul 1, 1988·The Journal of Investigative Dermatology·M L WilliamsP M Elias
Aug 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M PonecM L Williams
Aug 1, 1986·The Journal of Investigative Dermatology·K C MadisonD T Downing
May 1, 1974·Experimental Cell Research·S H Yuspa, C C Harris
Dec 1, 1994·Chemistry and Physics of Lipids·F Paltauf
Aug 1, 1994·In Vitro Cellular & Developmental Biology. Animal·H OkuI Chinen
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jun 24, 2004·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·K CouturierR Favier
Aug 1, 1994·In Vitro Cellular & Developmental Biology. Animal·H OkuI Chinen

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