Accumulation of 2-deoxy-D-glucose by primate oocytes fertilized in vitro

American Journal of Primatology
R J HutzW R Dukelow

Abstract

The present study was designed to investigate the accumulation of 2-deoxy-D-glucose (2-DG) by squirrel monkey oocytes fertilized in vitro; to assess the effects of insulin addition to the medium on 2-DG accumulation; and, finally, to evaluate the use of 2-DG in viability determinations of oocytes. Accumulation of 2-DG by unfertilized oocytes from squirrel monkeys was 13.94 fmol/oocyte/3 h and was not affected by the addition of either 10 nM or 1 μM insulin. There was no change in 2-DG accumulation with fertilization in vitro; 2-DG accumulation by degenerate ova was reduced to background levels. These results suggest low utilization of glucose by early primate embryos similar to that demonstrated for other mammalian species; 2-DG appears to be a good viability indicator of early primate embryos.

References

Mar 1, 1977·Journal of Reproduction and Fertility·M T Kane, N J Buckley
Jan 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·I W Davidson, W L Blackwell
Oct 1, 1965·Journal of Reproduction and Fertility·R L Brinster
Dec 7, 1981·Biochimica Et Biophysica Acta·P J Van den Broek, J Van Steveninck
Jan 1, 1980·Journal of Reproduction and Fertility·J P RenardY Menezo
Jul 16, 1980·Biochemical and Biophysical Research Communications·M Dunn, L Mallucci

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