Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat

The Plant Cell
J DanylukF Sarhan

Abstract

Expression of the acidic dehydrin gene wcor410 was found to be associated with the development of freezing tolerance in several Gramineae species. This gene is part of a family of three homologous members, wcor410, wcor410b, and wcor410c, that have been mapped to the long arms of the homologous group 6 chromosomes of hexaploid wheat. To gain insight into the function of this gene family, antibodies were raised against the WCOR410 protein and affinity purified to eliminate cross-reactivity with the WCS120 dehydrin-like protein of wheat. Protein gel blot analyses showed that the accumulation of WCOR410 proteins correlates well with the capacity of each cultivar to cold acclimate and develop freezing tolerance. Immunoelectron microscope analyses revealed that these proteins accumulate in the vicinity of the plasma membrane of cells in the sensitive vascular transition area where freeze-induced dehydration is likely to be more severe. Biochemical fractionation experiments indicated that WCOR410 is a peripheral protein and not an integral membrane protein. These results provide direct evidence that a subtype of the dehydrin family accumulates near the plasma membrane. The properties, abundance, and localization of these proteins sug...Continue Reading

Citations

May 5, 2007·Die Naturwissenschaften·Alan Tunnacliffe, Michael J Wise
Apr 21, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Vibeke AlmOdd Arne Rognli
Aug 30, 2011·Plant Cell Reports·Selvam AyarpadikannanJai-Heon Lee
Aug 8, 2012·Plant Cell Reports·Hernán Pablo BurriezaSara Maldonado
May 22, 2008·Planta·Igor A YakovlevOystein Johnsen
Dec 25, 2012·Protoplasma·Francine Lunardi Farias-SoaresMiguel Pedro Guerra
Dec 6, 2012·Molecular Biology Reports·Jie SunYongzhi Liu
Jan 11, 2007·Plant Molecular Biology·Debbie L Laudencia-ChingcuancoOlin D Anderson
Oct 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Paramjit KhuranaAnju K Chhibbar
Mar 10, 2015·Journal of Advanced Research·Nemat M HassanMamdouh M Nemat Alla
Oct 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Tomohiro ImamuraHideyuki Takahashi
Nov 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Yuezhi WangZhengqiang Ma
Feb 26, 1999·The Plant Journal : for Cell and Molecular Biology·V SantoniM Rossignol
Nov 8, 2007·Critical Reviews in Food Science and Nutrition·Federico Gómez GalindoKarl Kaack
Jul 9, 2004·Critical Reviews in Food Science and Nutrition·Federico Gómez GalindoIngegerd Sjöholm
Dec 27, 2011·Plant & Cell Physiology·Imen AmaraAdela Goday
Mar 24, 2012·Plant & Cell Physiology·Chia-Hui LinTsai-Yun Lin
Dec 18, 2013·Tree Physiology·Trygve Devold KjellsenG Richard Strimbeck
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·A M IsmailT J Close
May 13, 2010·Bioscience, Biotechnology, and Biochemistry·Faiçal BriniMoez Hanin
Oct 3, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Tsuyoshi TanakaHirokazu Handa
Jul 28, 2001·Physiologia Plantarum·Kaarina Pihakaski-MaunsbachArvid B. Maunsbach
Jun 30, 2014·Plant & Cell Physiology·Po-Hsin PengTsai-Yun Lin

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