PMID: 12009316May 16, 2002Paper

Accumulation of arachidonic acid-rich triacylglycerols in the microalga Parietochloris incisa (Trebuxiophyceae, Chlorophyta)

Phytochemistry
C BigognoZvi Cohen

Abstract

The freshwater green microalga Parietochloris incisa is the richest known plant source of the polyunsaturated fatty acid (PUFA), arachidonic acid (20:4omega6, AA). While many microalgae accumulate triacylglycerols (TAG) in the stationary phase or under certain stress conditions, these TAG are generally made of saturated and monounsaturated fatty acids. In contrast, most cellular AA of P. incisa resides in TAG. Using various inhibitors, we have attempted to find out if the induction of the biosynthesis of AA and the accumulation of TAG are codependent. Salicylhydroxamic acid (SHAM) affected a growth reduction that was accompanied with an increase in the content of TAG from 3.0 to 6.2% of dry weight. The proportion of 18:1 increased sharply in all lipids while that of 18:2 and its down stream products, 18:3omega6, 20:3omega6 and AA, decreased, indicating an inhibition of the Delta12 desaturation of 18:1. Treatment with the herbicide SAN 9785 significantly reduced the proportion of TAG. However, the proportion of AA in TAG, as well as in the polar lipids, increased. These findings indicate that while there is a preference for AA as a building block of TAG, the latter can be produced using other fatty acids, when the production of ...Continue Reading

References

Aug 14, 1999·Biochimica Et Biophysica Acta·I Khozin-GoldbergZ Cohen

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Citations

Jul 2, 2011·Applied Microbiology and Biotechnology·Inna Khozin-GoldbergZvi Cohen
Jul 19, 2011·Journal of Industrial Microbiology & Biotechnology·C Fuentes-GrünewaldJ Camp
Aug 17, 2010·Biochimie·Inna Khozin-Goldberg, Zvi Cohen
Feb 24, 2006·Progress in Lipid Research·Irina A Guschina, John L Harwood
Nov 26, 2014·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Nicolas J TourasseOlivier Vallon
Jun 8, 2017·BMC Plant Biology·Heike SieglerIvo Feussner
Jul 30, 2020·Biomolecules·Aleš TomčalaMiroslav Oborník

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