Accumulation of attenuating mutations in varying proportions within a high passage very virulent plus strain of Gallid herpesvirus type 2

Virus Research
Stephen J Spatz

Abstract

Marek's disease (MD) is controlled through mass vaccination. Although these vaccines reduce or delay tumor formation they fail to induce sterilizing immunity and prevent virus shedding. Relatively little is known about the genetic changes that lead to attenuation. It has been established that serial passage of virulent strains in avian cell lines results in the generation of attenuated progeny at some undefined passage level. A detailed cataloging of the mutations needed for attenuation will be important for advancing our understanding of MD biology and should facilitate the development of better vaccines. Using deep sequencing, the complete nucleotide sequence of the very virulent plus (vv+) strain 648A representing the 101 st passage was determined. Pathotyping studies have indicated that 648Ap101 is indeed attenuated. Comparative sequence analysis with the 648A strain at passage 11 has identified numerous gross genetic changes and subtle single nucleotide polymorphisms scattered throughout the genome. Relative to the strain (648Ap11), deletions were identified in MD-specific genes located in the repeat long (R(L)), unique long (U(L)) and repeat short (R(S)) regions. A deletion in the R(L) region, present in 33% of the p101 s...Continue Reading

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Citations

Jan 27, 2015·Avian Diseases·Grzegorz Woźniakowski, Elżbieta Samorek-SalamonowiczA
Jun 1, 2011·PLoS Pathogens·Nicholas RenzetteTimothy F Kowalik
Aug 2, 2013·Avian Diseases·Henry D Hunt, John R Dunn
Aug 28, 2012·Virus Genes·Stephen J SpatzRichard L Witter
Nov 3, 2010·Equine Veterinary Journal·S PronostU B R Balasuriya
Apr 30, 2015·Journal of Virological Methods·Grzegorz Woźniakowski, Jowita Samanta Niczyporuk
Oct 12, 2016·The Journal of Veterinary Medical Science·Yuka MachidaKazuhiko Ohashi
Jan 1, 2018·Current Clinical Microbiology Reports·Nikolaus Osterrieder, Dusan Kunec
Jan 24, 2021·Research in Veterinary Science·Zhaobo ZhangLiping Han
Jul 13, 2021·Journal of Veterinary Research·Katarzyna Pasik, Katarzyna Domańska-Blicharz

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