Accumulation of Cav 3.2 T-type Calcium Channels in the Uninjured Sural Nerve Contributes to Neuropathic Pain in Rats with Spared Nerve Injury

Frontiers in Molecular Neuroscience
Wen ChenFeng-Yu Liu

Abstract

Injuries to peripheral nerve fibers induce neuropathic pain. But the involvement of adjacent uninjured fibers to pain is not fully understood. The present study aims to investigate the possible contribution of Cav3.2 T-type calcium channels in uninjured afferent nerve fibers to neuropathic pain in rats with spared nerve injury (SNI). Aβ-, Aδ- and C-fibers of the uninjured sural nerve were sensitized revealed by in vivo single-unit recording, which were accompanied by accumulation of Cav3.2 T-type calcium channel proteins shown by Western blotting. Application of mibefradil, a T-type calcium channel blocker, to sural nerve receptive fields increased mechanical thresholds of Aβ-, Aδ- and C-fibers, confirming the functional involvement of accumulated channels in the sural nerve in SNI rats. Finally, perineural application of mibefradil or TTA-P2 to the uninjured sural nerve alleviated mechanical allodynia in SNI rats. These results suggest that axonal accumulation of Cav3.2 T-type calcium channels plays an important role in the uninjured sural nerve sensitization and contributes to neuropathic pain.

References

May 1, 1979·The Journal of Comparative Neurology·M DevorP D Wall
Jan 1, 1980·Annual Review of Pharmacology and Toxicology·W J Dixon
Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
Apr 1, 1993·Neuroreport·K M MearowJ Diamond
May 1, 1993·Journal of Neurophysiology·J W LeemJ M Chung
Dec 16, 1998·Neurobiology of Disease·H L FieldsR Baron
Apr 24, 1999·The European Journal of Neuroscience·M KoltzenburgS B McMahon
Jun 10, 2000·Science·C J Woolf, M W Salter
Feb 5, 2002·Journal of Neurophysiology·Esther M PogatzkiTimothy J Brennan
Dec 31, 2002·Physiological Reviews·Edward Perez-Reyes
Jun 17, 2003·Nature Neuroscience·Jung-Bum ShinGary R Lewin
Jul 30, 2005·Anesthesiology·Matthias Ringkamp, Richard A Meyer
Oct 4, 2006·Neuron·James N Campbell, Richard A Meyer
Dec 2, 2008·Trends in Pharmacological Sciences·Mircea C Iftinca, Gerald W Zamponi
Aug 29, 2009·The Journal of Pain : Official Journal of the American Pain Society·Alban Latremoliere, Clifford J Woolf
Apr 20, 2010·Journal of Neuroinflammation·Lawrence Leung, Catherine M Cahill
Nov 3, 2010·The Journal of Clinical Investigation·Adrienne E Dubin, Ardem Patapoutian
Dec 2, 2010·The Journal of Comparative Neurology·Jennifer C Peleshok, Alfredo Ribeiro-da-Silva
Feb 11, 2011·British Journal of Pharmacology·Slobodan M Todorovic, Vesna Jevtovic-Todorovic
Apr 14, 2011·The Journal of Physiology·Rui Wang, Gary R Lewin
Sep 21, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Min Jung KwonByung Gon Kim
Nov 25, 2014·Nature Neuroscience·Dmitry UsoskinPatrik Ernfors
Jan 21, 2015·Cell Reports·Amaury FrançoisEmmanuel Bourinet
Sep 12, 2015·Cold Spring Harbor Perspectives in Biology·Matthew N Rasband, Elior Peles
Sep 15, 2015·The Journal of Physiology·Zhongsheng Zhang, Gavriel David

❮ Previous
Next ❯

Citations

Jun 26, 2020·Physiological Reviews·Nanna Brix FinnerupTroels Staehelin Jensen
Jan 11, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Song CaiRajesh Khanna
Dec 29, 2020·Frontiers in Cellular Neuroscience·Emily L Tran, LaTasha K Crawford
Nov 13, 2020·Pharmacology & Therapeutics·Vipin AroraMan-Kyo Chung

❮ Previous
Next ❯

Software Mentioned

TotalLAB
Spike
GraphPad Prism
Spot Advanced
Photoshop
GraphPad

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

© 2022 Meta ULC. All rights reserved