Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
Beata KmiecPedro F Teixeira

Abstract

The stepwise degradation of peptides to amino acids in plant mitochondria and chloroplasts is catalyzed by a network of oligopeptidases (presequence protease PreP, organellar oligopeptidase OOP) and aminopeptidases. In the present report, we show that the lack of oligopeptidase activity in Arabidopsis thaliana results in the accumulation of endogenous free peptides, mostly of chloroplastic origin (targeting peptides and degradation products). Using mRNA sequencing and deep coverage proteomics, allowing for the identification of 17 000 transcripts and 11 000 proteins, respectively, we uncover a peptide-stress response occurring in plants lacking PreP and OOP oligopeptidase activity. The peptide-stress response results in the activation of the classical plant defense pathways in the absence of pathogenic challenge. The constitutive activation of the pathogen-defense pathways imposes a strong growth penalty and a reduction of the plants reproductive fitness. Our results indicate that the absence of organellar oligopeptidases PreP1/2 and OOP results in the accumulation of peptides that are perceived as pathogenic effectors and activate the signaling pathways of plant-defense response.

References

May 1, 1969·Stain Technology·M P Alexander
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·L Gómez-GómezT Boller
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·J F Martínez-GarcíaP H Quail
Feb 8, 2002·Trends in Plant Science·Keith LindseyPaul Chilley
Oct 10, 2002·FEBS Letters·Henrik Aronsson, Paul Jarvis
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Per MobergElzbieta Glaser
Nov 18, 2006·Nature Immunology·Brody J DeYoung, Roger W Innes
Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Viola A ManningLynda M Ciuffetti
Jun 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Keun Pyo LeeKlaus Apel
Mar 11, 2008·Cell Host & Microbe·Jeffrey CaplanSavithramma P Dinesh-Kumar
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Aug 25, 2009·Plant Molecular Biology·Stefan Nilsson CederholmElzbieta Glaser
Jan 1, 2008·Advances in Bioinformatics·Tomas HruzPhilip Zimmermann
Feb 12, 2011·Plant Physiology·Iris FinkemeierLee J Sweetlove
Apr 13, 2012·Biochimica Et Biophysica Acta·Pedro Filipe Teixeira, Elzbieta Glaser
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 10, 2013·Journal of Experimental Botany·Markus Albert
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Beata KmiecElzbieta Glaser
Nov 19, 2013·Nature Methods·Rui M M BrancaJanne Lehtiö
Apr 30, 2014·Molecular Plant·Bethany HuotSheng Yang He
Oct 13, 2014·Current Opinion in Microbiology·Verena ZimorskiSven B Gould
Oct 18, 2014·Journal of Experimental Botany·Monika W MurchaJames Whelan
Nov 22, 2014·Nucleic Acids Research·Vivek KrishnakumarChristopher D Town
Apr 26, 2015·Methods in Molecular Biology·Pedro F TeixeiraElzbieta Glaser
May 20, 2015·Molecular & Cellular Proteomics : MCP·Mikhail M SavitskiMarcus Bantscheff
Jan 1, 2015·Nature Plants·Marta de Torres ZabalaMurray Grant
Oct 18, 2016·Current Opinion in Structural Biology·Wen SongJijie Chai
Oct 21, 2016·Frontiers in Microbiology·Jinping ZhaoYule Liu
Nov 8, 2016·Nature Chemical Biology·Pedro F TeixeiraElzbieta Glaser

❮ Previous
Next ❯

Citations

Nov 14, 2018·The Plant Journal : for Cell and Molecular Biology·Sheila McCormick
Sep 8, 2019·International Journal of Molecular Sciences·Irina LyapinaIgor Fesenko
Nov 19, 2019·Journal of Experimental Botany·Abi S GhifariMonika W Murcha
Sep 23, 2020·The FEBS Journal·Friederike-Nora Vögtle
Jan 23, 2021·The Plant Journal : for Cell and Molecular Biology·Anthony A IannettaLeslie M Hicks

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.