Accumulation of hypoxia-inducible factor-1 alpha protein and its role in the differentiation of myeloid leukemic cells induced by all-trans retinoic acid

Haematologica
Jing ZhangGuo-Qiang Chen

Abstract

The clinical activities of all-trans retinoic acid in the treatment of acute promyelocytic leukemia, a unique subtype of acute myeloid leukemia, have triggered extensive studies aimed at defining the mechanisms by which this compound induces differentiation of leukemic cells. Recent studies show that hypoxia-inducible factor-1 alpha (HIF-1 alpha) contributes to the differentiation of acute myeloid leukemia cells via transcriptional activity-independent mechanisms. We investigated whether all-trans retinoic acid affects HIF-1 alpha protein and whether this has a role in all-trans retinoic acid-induced differentiation. The acute myeloid leukemia cell lines NB4 and U937 were treated with all-trans retinoic acid, and HIF-1 alpha/HIF-1 beta mRNA and proteins were measured respectively by real-time quantitative reverse transcriptase polymerase chain reaction and western blotting. To investigate the role of HIF-1 alpha in all-trans retinoic acid-induced differentiation, NB4 cells, U937 cells, U937 cells in which HIF-1 alpha was induced by the withdrawal of tetracycline and U937 cells with stable expression of specific short hairpin RNA against HIF-1 alpha, Runx1, C/EBP alpha and PU.1, were treated with all-trans retinoic acid and/or t...Continue Reading

Citations

May 1, 2013·Genes & Development·Julie-Aurore Losman, William G Kaelin
Jun 19, 2012·Seminars in Cancer Biology·Chuan Shen, William G Kaelin
Jun 12, 2014·Science China. Life Sciences·Jie LiGuoQiang Chen
Aug 25, 2010·Pharmacology & Therapeutics·Gabriella LeonarduzziGiuseppe Poli
Mar 10, 2016·Frontiers in Oncology·Margaux DeynouxFrédéric Mazurier
Sep 25, 2009·Leukemia Research·Mario P TschanMartin F Fey
Mar 17, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Jing Zhang, Guo-Qiang Chen
Dec 25, 2012·Biochemical and Biophysical Research Communications·Zhao-Wen YanYing Huang
Dec 10, 2009·Biochemical and Biophysical Research Communications·Aurelia Walczak-DrzewieckaJarosław Dastych
Mar 13, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Diogo TrigoJonathan P T Corcoran
May 11, 2011·The International Journal of Biochemistry & Cell Biology·Ana Belén Fernández-MartínezFrancisco Javier de Lucio-Cazaña

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