Accumulation of lipochitin oligosaccharides and NodD-activating compounds in an efficient plant--Rhizobium nodulation assay

Molecular Plant-microbe Interactions : MPMI
T TakKees J M Boot

Abstract

During legume plant--Rhizobium spp. interactions, leading to the formation of nitrogen-fixing root nodules, the two major determinants of host plant-specificity are plant-produced nod gene inducers (NodD protein activating compounds) and bacterial lipochitin oligosaccharides (LCOs or Nod factors). In a time course, we describe the accumulation of LCOs in an efficient nodulation assay with Vicia sativa subsp. nigra and Rhizobium leguminosarum, in connection with the presence of NodD-activating compounds in the exudate of V. sativa roots. Relatively small amounts of both LCOs and NodD-activating compounds were found to be required for initiation of nodulation during the first days after inoculation. A strong increase in the amount of NodRlv-V[18:4,Ac] LCOs preceded root infection and nodule primordium formation. In contrast to the situation with non-nodulating rhizobia and nonmitogenic LCOs, the amount of NodD-activating compounds in the culture medium remained small after addition of nodulating rhizobia or mitogenic LCOs. Furthermore, addition of nodulating rhizobia or mitogenic LCOs resulted in nearly complete inhibition of root hair formation and elongation, whereas nonmitogenic LCOs stimulated root hair growth. Retention of N...Continue Reading

References

Jan 1, 1992·Molecular Plant-microbe Interactions : MPMI·H P SpainkE P Kennedy
Oct 12, 2000·Plant Physiology·J Stougaard
Feb 13, 2001·Trends in Plant Science·J V CullimoreJ J Bono
May 30, 2001·Current Biology : CB·J Mathur, M Hülskamp
May 25, 2002·Molecular Plant-microbe Interactions : MPMI·Anton A N van BrusselJan W Kijne
Dec 7, 2002·The Plant Cell·Hyung-Taeg Cho, Daniel J Cosgrove
Feb 13, 2003·Molecular Plant-microbe Interactions : MPMI·Paulina C van SpronsenKees J M Boot
Jan 1, 1989·Plant Molecular Biology·H P SpainkB E Lugtenberg
Jan 1, 1987·Plant Molecular Biology·H P SpainkB J Lugtenberg

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Citations

Apr 17, 2009·Journal of Chemical Ecology·Dominika MajAnna Skorupska
Jan 1, 2010·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·D V KambojP K Sharma

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