Accumulation of Mannosylglycerate and Di-myo-Inositol-Phosphate by Pyrococcus furiosus in Response to Salinity and Temperature.

Applied and Environmental Microbiology
L O Martins, H Santos

Abstract

(sup13)C and (sup1)H nuclear magnetic resonance spectroscopy was used to identify and quantify organic solutes accumulated by the hyperthermophilic archaeon Pyrococcus furiosus in response to temperature and salinity. Di-myo-inositol-phosphate and 2-O-(beta)-mannosylglycerate were the major organic solutes accumulated in these cells. The total intracellular organic solutes increased significantly in response either to an increase in temperature or to an increase in salinity, but (beta)-mannosylglycerate accumulated mainly at high salinities, whereas the concentration of di-myo-inositol-phosphate increased dramatically at supraoptimal growth temperatures. Glutamate was present at concentrations detectable by nuclear magnetic resonance only in cells grown in low-salinity media. The intracellular levels of K(sup+) are clearly dependent on the salinity of the medium, and the concentrations of this cation are high enough to counterbalance the negative charges of (beta)-mannosylglycerate and di-myo-inositol-phosphate in the cell. The results presented here together with those previously reported for Pyrococcus woesei (S. Scholz, J. Sonnenbichler, W. Schafer, and R. Hensel, FEBS Lett. 306:239-242, 1992) strongly support a role for di-...Continue Reading

References

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Jun 1, 1995·Applied and Environmental Microbiology·O C NunesH Santos

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Citations

Sep 8, 2006·Applied and Environmental Microbiology·Pedro LamosaHelena Santos
Jun 22, 2010·Journal of Bacteriology·Ana-Maria SevcencoPeter-Leon Hagedoorn
Jun 24, 2015·Extremophiles : Life Under Extreme Conditions·Mohamed JebbarPhilippe Oger
Jan 18, 2017·Archives of Microbiology·Preeti Ranawat, Seema Rawat
Sep 11, 2002·Environmental Microbiology·Helena Santos, Milton S da Costa
Sep 1, 2009·Metallomics : Integrated Biometal Science·Ana-Maria SevcencoWilfred R Hagen
Oct 15, 2013·FEMS Microbiology Reviews·Florian Mayer, Volker Müller
Nov 16, 2011·Molecular Microbiology·Kerstin LassakSonja-Verena Albers
Apr 22, 2011·Extremophiles : Life Under Extreme Conditions·Pedro LamosaGarabed Antranikian
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Nuno BorgesHelena Santos
Jul 4, 2020·Environmental Microbiology·Josephine Joy HubloherVolker Müller
Feb 15, 2003·FEMS Microbiology Letters·Luís G GonçalvesHelena Santos
Dec 5, 2012·Archaea : an International Microbiological Journal·Kimberly M Webb, Jocelyne DiRuggiero
Dec 14, 1999·Protein Science : a Publication of the Protein Society·T C ChengS I Chan
Feb 16, 2002·Environmental Microbiology·M Roesser, V Müller
Feb 21, 2008·Extremophiles : Life Under Extreme Conditions·Luís G GonçalvesHelena Santos
Oct 1, 1996·Protein Science : a Publication of the Protein Society·R SternerK Kirschner
Apr 5, 2003·Applied and Environmental Microbiology·Keith R ShockleyRobert M Kelly
Sep 8, 2006·Applied Microbiology and Biotechnology·Georg Lentzen, Thomas Schwarz

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