Accumulation of nuclear and mitochondrial DNA damage in the frontal cortex cells of patients with HIV-associated neurocognitive disorders

Brain Research
Yulin ZhangDexi Chen

Abstract

Oxidative stress has been suggested to play a key role in the neuropathogenesis of HIV infection. HIV proteins (gp120, Tat) and proinflammatory cytokines can trigger the production of reactive oxygen species (ROS), resulting in DNA and RNA lesions. Among all the lesions induced by ROS, one of the most abundant lesions in DNA and RNA is 8-hydroxydeoxyguanosine (8-oxoG). Here, we studied accumulated DNA oxidative damage induced by ROS in the central nervous system (CNS) in tissue from neuro-AIDS patients. The frontal cortex of autopsy tissue from HIV-1 infected patients was adopted for analysis for HIV-1 subtype, nuclear and mitochondrial DNA lesions by immunofluorescence staining, qPCR and sequencing of PCR cloning. This study provides evidence that HIV infection in the CNS leads to nuclear and mitochondrial genomic DNA damage in the brain. High level of nuclear and mtDNA 8-oxoG damage were identified in the cortex autopsy tissue of HAND patients. Increased accumulation of mtDNA mutations and depletion occurs in brain tissue in a subset of HAND cases, and is significantly different from that observed in control cases. These findings suggest that higher level of ROS in the CNS of HAND patients would contribute to the HIV induced ...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C G FragaB N Ames
May 1, 1993·Journal of Neurology, Neurosurgery, and Psychiatry·I P EverallP L Lantos
Nov 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A BassiriM Wong
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·M Kaul, S A Lipton
Feb 13, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·C ElbimM A Gougerot-Pocidalo
Apr 6, 2002·Journal of Neurovirology·Ned SacktorLeon Epstein
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Scott M RaidelWilliam Lewis
Jun 29, 2002·Science·Joan Kaufman, Jun Jing
Aug 4, 2004·Toxicology and Applied Pharmacology·Leonard W VelsorBrian J Day
Apr 6, 2005·The Journal of Infectious Diseases·Miriam CasulaAnthony de Ronde
May 4, 2005·Biochemical and Biophysical Research Communications·Gwenn A Garden, Richard S Morrison
May 14, 2005·Brain Research. Brain Research Reviews·Chava B PocernichD Allan Butterfield
Jul 30, 2005·Journal of Biosciences·Hakan Ozdener
Jan 20, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lauren A O'DonnellDennis L Kolson
Feb 18, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Celeste M BolinFernando Cardozo-Pelaez
Jun 8, 2006·Environmental and Molecular Mutagenesis·James J Kohler, William Lewis
Jun 10, 2006·Science·Zunyou WuRoger Detels
Jul 18, 2006·Current HIV Research·Marcus Kaul, Stuart A Lipton
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eliseo A EugeninJoan W Berman
Apr 6, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gareth J JonesChristopher Power
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Marcus Kaul
Dec 20, 2007·Journal of Neurochemistry·Changhai TianJialin Zheng
Jun 3, 2008·Biotechnology Journal·Amy K ReeveDoug M Turnbull
Nov 11, 2008·Journal of Neurovirology·Robert K HeatonUNKNOWN San Diego HIV Neurobehavioral Research Center Group
Nov 27, 2009·Accounts of Chemical Research·Sriram KanvahUzi Landman
Mar 4, 2011·AIDS Research and Human Retroviruses·Feili WeiDexi Chen

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Citations

Dec 24, 2013·Virology Journal·Asen Bagashev, Bassel E Sawaya
Nov 28, 2013·Journal of Health Care for the Poor and Underserved·Melissa Agsalda-GarciaValerie Wojna
Aug 27, 2013·Critical Reviews in Immunology·Alexander J Gill, Dennis L Kolson
Feb 26, 2014·Journal of Neurovirology·Jennifer M CoughlinMartin G Pomper
Apr 4, 2014·Journal of Molecular Neuroscience : MN·Summer J RozziValeriya Avdoshina
Sep 28, 2015·Cytokine & Growth Factor Reviews·Xavier Dagenais-LussierJulien van Grevenynghe
Jul 2, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Todd HulganUNKNOWN CHARTER Group
Nov 11, 2016·Oxidative Medicine and Cellular Longevity·Alexander V IvanovMaria G Isaguliants
Jan 23, 2017·Current HIV/AIDS Reports·Jacqueline ElleroBruce Brew
Dec 28, 2017·Journal of Neurovirology·Ronald A CohenM-Z Ding
Jul 6, 2014·Current HIV/AIDS Reports·Asha R Kallianpur, Andrew J Levine
Jul 12, 2017·Neurotoxicity Research·Summer J RozziItalo Mocchetti
Nov 18, 2018·International Journal of Molecular Sciences·Ian Simon OlivierVinogran Naidoo
Sep 19, 2019·Archives of Virology·Agieshkumar Balakrishna PillaiSoundravally Rajendiran
Jun 19, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Luc BertrandMichal Toborek
Feb 7, 2021·Biology·Moses New-AaronNatalia A Osna
Nov 13, 2020·The Journal of Antimicrobial Chemotherapy·Gilles ForceUNKNOWN NEURO+3 STUDY GROUP
Oct 16, 2020·Neurology. Neuroimmunology and Neuroinflammation·Ronald J EllisScott L Letendre
Jun 25, 2019·The American Journal of Pathology·Swati ShahWilliam Reid
Jul 25, 2021·Archives of Pharmacal Research·Young-Jung LeeJin Tae Hong

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