PMID: 39599Oct 10, 1979

Accumulation of pyruvate by isolated rat liver mitochondria

Biochimica Et Biophysica Acta
W J VaartjesS G van den Bergh

Abstract

1. Various methods to measure the rate of accumulation of [3-14C]pyruvate in the sucrose-impermeable space of isolated rat liver mitochondria are tested and compared with respect to their ability to distinguish between carrier-linked pyruvate transport and non-carrier-linked processes (adsorption and diffusion). 2. Evidence is presented that the cinnamic acid derivatives commonly used as specific inhibitors of the pyruvate carrier (i) do not completely abolish all carrier-mediated pyruvate transport; (ii) inhibit pyruvate adsorption, and (iii) at higher concentrations lead to a removal of previously accumulated pyruvate from the mitochondria. It is concluded that procedures which avoid the use of transport inhibitors allow more reliable estimates of carrier-linked pyruvate transport. 3. It is proposed to measure pyruvate adsorption as the accumulation of pyruvate in the presence of an uncoupler. Using this procedure, it could be shown that, with 1 mM pyruvate, adsorption represents only a small part of the total pyruvate accumulation, the main part being carrier-linked transport driven by the pH gradient across the mitochondrial inner membrane.

References

May 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·B C Pressman

Citations

Dec 1, 1982·Mechanisms of Ageing and Development·D R Deshmukh, M S Patel
Aug 17, 1988·Biochimica Et Biophysica Acta·G Paradies, F M Ruggiero
Feb 1, 1982·European Journal of Biochemistry·A K GroenJ M Tager
Jun 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P F FosterJ W Williams

Related Concepts

Adsorption
Metazoa
Bacteriocidal Agents
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Cinnamates
Coumaric Acids
Diffusion
Hydrogen-Ion Concentration
Mitochondria, Liver
Pandavir

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