PMID: 8965656Mar 1, 1996Paper

Accumulation of S100 beta mRNA and protein in cerebellum during infancy in Down syndrome and control subjects

Brain Research. Molecular Brain Research
A MarksL E Becker

Abstract

S100 protein is a 20 kDA calcium-binding protein that accumulates during CNS maturation in mammals. The human gene coding for the beta subunit of S100 protein (S100 beta) is located on chromosome 21, in a subtelomeric position in 21q22.3. In order to investigate the effect of trisomy 21 on S100 beta gene expression, we performed Southern, Northern and Western blot analysis on DNA, RNA and protein, respectively, extracted from the cerebellum of control and Down syndrome (DS) subjects aged 1-18 months. Southern blot analysis revealed a novel EcoRI polymorphism in the S100 beta gene in two of 15 DNA samples examined, and a 1.5 gene dosage for S100 beta in DS. Northern and Western blot analysis showed an approximately 10-fold increase in S100 beta mRNA and protein levels between 1 and 18 months. No differences in the rates of accumulation of S100 beta mRNA and protein were observed between DS and normal subjects. These results demonstrate an increase in S100 beta mRNA and protein levels during infancy indicative of postnatal astrocytic maturation and show that there is no gross deregulation in the expression of the S100 beta gene in DS as a consequence of trisomy 21.

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Citations

Feb 17, 2000·Brain Research. Developmental Brain Research·S C TiuD T Yew
Oct 24, 2002·Brain Research. Molecular Brain Research·Richard H DyckG Campbell Teskey
Mar 20, 2003·Microscopy Research and Technique·Matthias RothermundtVolker Arolt
Sep 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Cristina B O NettoRoberto Giugliani
Jan 25, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jinger do Carmo CunhaGerson Chadi
Apr 8, 2010·Free Radical Research·Irene PaternitiSalvatore Cuzzocrea
Mar 27, 2001·Journal of Developmental and Behavioral Pediatrics : JDBP·G T Capone
Apr 13, 2006·IUBMB Life·Francesca ArgellatiRoberta Ricciarelli
May 6, 2008·Clinical Biochemistry·Carlos-Alberto GonçalvesPatrícia Nardin

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