PMID: 708736Sep 27, 1978Paper

Accumulation of small fragments of DNA in isolated HeLa cell nuclei due to transient incorporation of dUMP

Biochimica Et Biophysica Acta
E WistH Krokan

Abstract

[3H]dUMP was incorporated into DNA of isolated S-phase HeLa S3 cell nuclei during DNA synthesis. The incorporated radioactivity was made acid soluble during a chase with excess TTP. A partially purified DNA polymerase alpha incorporated [3H]dUMP into activated salmon sperm DNA. The incorporation rate was equal to the incorporation of [3H]TMP, and the radioactivity incorporated was not made acid soluble during a chase. The nuclei thus have the ability to remove misincorporated uracil. From cytosol we have partially purified an enzyme (80 times purification) that splits the N-glycosidic bond between uracil and deoxyribose in dUMP-containing DNA. This uracil-N-glycosidase has a molecular weight of about 50 000. It does not accept dUTP or RNA as substrates. Pulse labelling of isolated nuclei with radioactive deoxyribonucleoside triphosphates in the presence of dUTP lead to a large accumulation of label in small DNA fragments. The size of these fragments was about 80 nucleotides in a 60 s pulse and no increase in size was observed with increasing pulse length. The corresponding value for control experiments with no dUTP, was 200 nucleotides and the fragments increased in size with increasing pulse length. About 90% of the radioactiv...Continue Reading

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B K TyeB Weiss
Mar 21, 1977·Biochemical and Biophysical Research Communications·H KrokanH Prydz
Jan 1, 1975·Annual Review of Biochemistry·M L Gefter
Mar 28, 1969·Science·A Kornberg
Jul 30, 1974·Biochemistry·T Lindahl, B Nyberg
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·T Lindahl
Aug 20, 1971·Biochemical and Biophysical Research Communications·A SchlabachA Weissbach
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER
Jul 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·M J BessmanA Kornberg

Citations

Jan 1, 1980·Advances in Enzyme Regulation·W D Sedwick, J Laszlo
Jan 1, 1984·Advances in Enzyme Regulation·R G RichardsW D Sedwick
Jun 9, 2005·Mutation Research·Joy L HuffmanJohn A Tainer
Dec 6, 2000·Mutation Research·K KvaløyHans Einar Krokan
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M GoulianB Y Tseng
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W D SedwickO E Brown
Jan 1, 1980·Experimental Cell Research·S MahagaokarP N Rao
Mar 30, 1979·Biochemical and Biophysical Research Communications·H Ariga, H Shimojo
Apr 22, 2014·DNA Repair·Hans Einar KrokanGeir Slupphaug
Aug 8, 2009·FEMS Microbiology Reviews·Bjørn DalhusMagnar Bjørås
Sep 1, 1979·FEBS Letters·P Reinhard, M Schluchter
Dec 15, 1982·Biochemical and Biophysical Research Communications·H A Ingraham, M Goulian
Jan 1, 1988·Environmental and Molecular Mutagenesis·S S Wallace
Jun 1, 1980·Clinical and Experimental Dermatology·F Giannelli
Dec 17, 2002·Oncogene·Hans Einar KrokanGeir Slupphaug
Feb 13, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hua ZhangRuqin Yu
Aug 1, 1993·Physiologia Plantarum·Atle M Bones
May 1, 2021·International Journal of Molecular Sciences·Ryszard OlinskiHans Einar Krokan