Accumulation of small protein molecules in a macroscopic complex coacervate

Soft Matter
Saskia Lindhoud, Mireille M A E Claessens

Abstract

To obtain insight into the accumulation of proteins into macroscopic complex coacervate phases, the lysozyme concentration in complex coacervates containing the cationic polyelectrolyte poly-(N,N dimethylaminoethyl methacrylate) and the anionic polyelectrolyte polyacrylic acid was investigated as a function of the mixing ratio, protein concentration and ionic strength. Maximal protein enrichment of the complex coacervate phase was observed to require the presence of all three macromolecules. Under optimized conditions the protein concentrations in the complex coacervate were as high as 200 g L(-1). Such high concentrations are comparable to the protein concentration in the cytosol, suggesting that these interesting liquid phases may serve a suitable model system for the phase behavior of the cytosol and genesis and function of membrane-less organelles. The high stability of the complexes and the salt dependent uptake of protein suggest that complex coacervates may provide a way to store hydrated proteins at high concentrations and might therefore be of interest in the formulation of high protein foods.

References

Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
Oct 9, 2001·Trends in Biochemical Sciences·R J Ellis
Mar 11, 2004·The European Physical Journal. E, Soft Matter·J-F BerretI Grillo
Jul 13, 2004·Biomacromolecules·F WeinbreckC G de Kruif
Jul 21, 2004·Colloids and Surfaces. B, Biointerfaces·Marijn van der VeenMartien Cohen Stuart
Apr 5, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Stefan van der BurghMartien A Cohen Stuart
Dec 8, 2006·The Journal of Physical Chemistry. B·Jérémie GummelFabrice Cousin
May 29, 2007·Biomacromolecules·Saskia LindhoudMartien A Cohen Stuart
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Dirar HomouzPernilla Wittung-Stafshede
Nov 29, 2008·Advances in Colloid and Interface Science·Ilja K VoetsMartien A Cohen Stuart
Apr 2, 2009·The Journal of Physical Chemistry. B·Saskia LindhoudMartien A Cohen Stuart
Aug 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Saskia LindhoudWillem Norde
Apr 15, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Saskia LindhoudMartien A Cohen Stuart
Dec 26, 2013·The Journal of Cell Biology·Clifford P Brangwynne
Sep 3, 2014·The Journal of Cell Biology·Jeffrey A Toretsky, Peter E Wright
May 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Shana Elbaum-GarfinkleClifford P Brangwynne

❮ Previous
Next ❯

Citations

Sep 24, 2016·Angewandte Chemie·David GarenneJean-Paul Douliez
Sep 5, 2017·Organic & Biomolecular Chemistry·Brandon M JohnstonSarah L Perry
Apr 21, 2018·Chembiochem : a European Journal of Chemical Biology·Naoki NakataniKenichi Yoshikawa
Mar 28, 2019·Soft Matter·Whitney C Blocher McTigue, Sarah L Perry
May 1, 2019·Chembiochem : a European Journal of Chemical Biology·Nicolas Martin
Jul 3, 2019·Polymers·Shang GaoSamanvaya Srivastava
Mar 7, 2020·Soft Matter·Charles E Sing, Sarah L Perry
May 5, 2020·Small·Whitney C Blocher McTigue, Sarah L Perry
Sep 9, 2020·Physical Chemistry Chemical Physics : PCCP·Whitney C Blocher McTigueSarah L Perry
Nov 6, 2018·The Journal of Chemical Physics·Mengmeng Zhao, Nicole S Zacharia
Dec 5, 2017·Soft Matter·Karina K NakashimaEvan Spruijt
Apr 20, 2019·Frontiers in Molecular Biosciences·Karina K NakashimaEvan Spruijt
Nov 5, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Whitney C Blocher, Sarah L Perry
Apr 19, 2019·Chemical Science·Rachel A Kapelner, Allie C Obermeyer
Jul 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Henry Shum, Anna C Balazs
Nov 10, 2020·Advanced Biosystems·Wenjin XiaoAdam W Perriman
Oct 21, 2020·Biomaterials Science·Xue MiSarah L Perry
Apr 12, 2018·Biophysical Journal·Patrick M McCallMatthew V Tirrell
Jun 22, 2021·Soft Matter·Nicholas A Zervoudis, Allie C Obermeyer
Jul 31, 2021·The Journal of Physical Chemistry. B·Ashley R KnoerdelCharles E Sing
Apr 4, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Hairong JingDehai Liang
Jul 1, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Riahna KembarenJan Willem Borst
Jun 26, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Hairong JingDehai Liang
Aug 31, 2021·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Jéré van LenteSaskia Lindhoud

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Langmuir : the ACS Journal of Surfaces and Colloids
Mahboubeh M Nejati, Morteza G Khaledi
Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
S ChodankarA G Wagh
The Journal of Pharmacy and Pharmacology
O N Singh, J Burgess
© 2021 Meta ULC. All rights reserved