Accumulation of soluble carbohydrates, trehalase and sucrose synthase in effective (Fix+) and ineffective (Fix-) nodules of soybean cultivars that differentially nodulate with Bradyrhizobium japonicum

Functional Plant Biology : FPB
Zhi-Ping XieJoachim Müller

Abstract

Roots of soybeans have the ability to form symbioses with nitrogen-fixing rhizobial bacteria to form nitrogen-fixing (Fix+) nodules, thus allowing the plant to grow in the absence of mineral nitrogen. Several soybean cultivars from China nodulated normally with Bradyrhizobium japonicum USDA110 spc4, but developed only a few nodules with 61-A-101, another B. japonicum strain. When soybeans were infected with Rhizobium sp. NGR234, ineffective (Fix-) nodules that do not fix nitrogen were formed. Plants infected with NGRΩnodD2, a mutant strain overproducing lipo-chitooligosaccharidic nodulation signals (Nod factors), showed significantly higher numbers of ineffective nodules. Nodules from the different plant-microsymbiont combinations were characterized with respect to their accumulation of soluble carbohydrates and their induction of trehalase and sucrose synthase. These two plant enzymes are known to be nodule-stimulated proteins. Pool sizes of soluble carbohydrates in nodules showed strain-specific alterations in sucrose and trehalose, whereas myo-inositol and pinitol were affected in a more cultivar-specific way. Immunoblots with nodulin-specific antiserum indicated that sucrose synthase is induced in Fix+ nodules, but undetect...Continue Reading

Citations

Mar 22, 2014·The Plant Journal : for Cell and Molecular Biology·John Edward LunnMark Stitt
Nov 6, 2012·The New Phytologist·Aarón BarrazaFederico Sanchez
Jun 1, 2007·Functional Plant Biology : FPB·John E Lunn
May 10, 2013·Plant Signaling & Behavior·Aarón Barraza, Federico Sánchez

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