Accumulation of tetracycline resistance genes in aquatic biofilms due to periodic waste loadings from swine lagoons

Environmental Science & Technology
Wen ZhangDavid W Graham

Abstract

Antibiotic resistance genes (ARGs) are emerging contaminants found in the water and sediments surrounding animal feedlots. In this study, the fate of five tetracycline-resistance and 16S-rRNA genes released in swine waste were monitored for 21 days in the water column and biofilms in 12 mesocosms mimicking different natural receiving water bodies. Four treatments were employed in triplicate: two light exposures (light/ dark) and two loading scenarios (single/periodic). As seen previously, light exposure had a significant effect on disappearance rates of tet genes in both the water column and biofilms, although absolute rates were significantly lower in the biofilms. Further, periodic versus single loading events resulted in >2 orders of magnitude higher tet gene levels in associated tanks. Regardless of treatment, ARGs migrated quickly to biofilms, with 3% and >85% of detected tet determinants found in biofilms on days 1 and 4, respectively. Overall, these are the first quantitative data on specific ARG disappearance rates in biofilms, and also the first evidence of progressively accumulating ARG levels in biofilms under loading conditions typical of natural receiving waters. In summary, ARGs migrate rapidly to biofilms where t...Continue Reading

References

Sep 21, 2001·Environmental Science & Technology·J Tolls
Feb 5, 2003·Environmental Science & Technology·Gerda HarmsGary S Sayler
Jun 19, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David W Hecht
Dec 3, 2004·Applied and Environmental Microbiology·Marilyn S SmithDavid W Graham
Feb 5, 2005·Applied and Environmental Microbiology·B S McSwainP A Wilderer
Sep 19, 2006·FEMS Microbiology Letters·Christina A EngemannDavid W Graham
Dec 22, 2006·Environmental Science & Technology·Amy PrudenKenneth H Carlson
Jan 24, 2007·Water Research·Eric A AuerbachKatherine D McMahon
Aug 23, 2007·Environmental Science & Technology·Ruoting PeiAmy Pruden
Aug 30, 2008·Environmental Science & Technology·Christina A EngemannDavid W Graham
Apr 29, 2009·Journal of Environmental Quality·Joanne C Chee-SanfordRustam I Aminov

❮ Previous
Next ❯

Citations

Oct 26, 2010·Animal Health Research Reviews·Mario JacquesYannick D N Tremblay
Jun 19, 2014·Environmental Science and Pollution Research International·C DomínguezJ M Bayona
Jun 5, 2012·Journal of Environmental Sciences (China)·Yong ChenChun Hu
Apr 5, 2011·Trends in Ecology & Evolution·Nick G H TaylorCraig Baker-Austin
Aug 7, 2013·Journal of Microbiological Methods·Wen ZhangM Katherine Banks
Jul 31, 2016·Environmental Pollution·Luis G Chaves-BarqueroCharles S Wong
Nov 20, 2015·Frontiers in Microbiology·José L BalcázarCarles M Borrego
Feb 9, 2017·Journal of Analytical Methods in Chemistry·Fabio Granados-Chinchilla, César Rodríguez
Nov 26, 2020·Environmental Pollution·Lianxue WeiJinfeng Lu
Dec 17, 2010·Environmental Science & Technology·Manu TamminenMarko Virta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.