Accumulation of Trichothecenes in Liquid Cultures of a Fusarium sporotrichioides Mutant Lacking a Functional Trichothecene C-15 Hydroxylase.

Applied and Environmental Microbiology
Susan P McCormick, T M Hohn

Abstract

A mutant strain of Fusarium sporotrichioides NRRL 3299 produced by disruption of Tri11, a gene encoding a cytochrome P-450 monooxygenase, was shown to be altered in its ability to biosynthesize T-2 toxin. This mutant strain produced four trichothecenes that were not observed in cultures of the parent strain. The compounds were identified as isotrichodermin, 8-hydroxyisotrichodermin, 8-hydroxyisotrichodermol, and 3,4,8-trihydroxytricothecene on the basis of their nuclear magnetic resonance and mass spectra. This is the first report of these 8-hydroxytrichothecenes as metabolites of F. sporotrichioides. The accumulation of isotrichodermin and the results of whole-cell feeding experiments with a Tri11(sup-) strain confirm that oxygenation of C-15 is blocked.

References

May 1, 1992·Molecular Plant-microbe Interactions : MPMI·T M Hohn, A E Desjardins
Mar 1, 1990·Applied and Environmental Microbiology·S P McCormickM N Beremand
Sep 1, 1989·Applied and Environmental Microbiology·R D PlattnerM N Beremand
Sep 1, 1989·Applied and Environmental Microbiology·S P McCormickM N Beremand
Sep 1, 1993·Microbiological Reviews·A E DesjardinsS P McCormick
Feb 1, 1996·Applied and Environmental Microbiology·S P McCormickA E Desjardins
Mar 1, 1986·Applied and Environmental Microbiology·A E DesjardinsF Vanmiddlesworth

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Citations

Aug 19, 2006·Canadian Journal of Microbiology·Susan P McCormickRobert H Proctor
Apr 11, 2006·Canadian Journal of Microbiology·Susan P McCormickRobert H Proctor
Apr 1, 2009·International Journal of Molecular Sciences·Nora A Foroud, François Eudes
Dec 20, 2008·Canadian Journal of Microbiology·Nancy J AlexanderJudith A Blackburn
Apr 9, 2015·Medicinal Research Reviews·Alexander KornienkoRobert Kiss
Jun 1, 2002·Applied and Environmental Microbiology·Susan P McCormick, Nancy J Alexander
Apr 7, 2004·Applied and Environmental Microbiology·S P McCormickA E Desjardins
Sep 11, 2007·Bioscience, Biotechnology, and Biochemistry·Makoto KimuraMakoto Fujimura
Dec 3, 1999·Applied and Environmental Microbiology·S P McCormickT M Hohn
Jul 17, 2007·Journal of Agricultural and Food Chemistry·Anne E DesjardinsMichael Appell

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