Accuracy, limitations and cost efficiency of eDNA-based community survey in tropical frogs

Molecular Ecology Resources
Miklós BálintMartin Jansen

Abstract

Rapid environmental change in highly biodiverse tropical regions demands efficient biomonitoring programmes. While existing metrics of species diversity and community composition rely on encounter-based survey data, eDNA recently emerged as alternative approach. Costs and ecological value of eDNA-based methods have rarely been evaluated in tropical regions, where high species richness is accompanied by high functional diversity (e.g., the use of different microhabitats by different species and life stages). We first tested whether estimation of tropical frogs' community structure derived from eDNA data is compatible with expert field assessments. Next, we evaluated whether eDNA is a financially viable solution for biodiversity monitoring in tropical regions. We applied eDNA metabarcoding to investigate frog species occurrence in five ponds in the Chiquitano dry forest region in Bolivia and compared our data with a simultaneous visual and audio encounter survey (VAES). We found that taxon lists and community structure generated with eDNA and VAES correspond closely, and most deviations are attributable to different species' life histories. Cost efficiency of eDNA surveys was mostly influenced by the richness of local fauna and t...Continue Reading

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Citations

Aug 14, 2020·Molecular Ecology·Carla Martins LopesKelly Raquel Zamudio
May 21, 2020·Evolutionary Applications·Maciej PabijanWiesław Babik
Mar 12, 2021·The Journal of Animal Ecology·Wenhao LiYiming Li
Mar 30, 2021·Ecological Applications : a Publication of the Ecological Society of America·José Luis MenaArnaud Lyet
Dec 14, 2021·PeerJ·Léo CharvozJan Pawlowski

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