Accuracy of digital mRNA profiling of oesophageal biopsies as a novel diagnostic approach to eosinophilic oesophagitis

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
W S LexmondS Nurko

Abstract

Quantification of tissue eosinophils remains the golden standard in diagnosing eosinophilic oesophagitis (EoE), but this approach suffers from poor specificity. It has been recognized that histopathological changes that occur in patients with EoE are associated with a disease-specific tissue transcriptome. We hypothesized that digital mRNA profiling targeted at a set of EoE-specific and Th2 inflammatory genes in oesophageal biopsies could help differentiate patients with EoE from those with reflux oesophagitis (RE) or normal tissue histology (NH). The mRNA expression levels of 79 target genes were defined in both proximal and distal biopsies of 196 patients with nCounter® (Nanostring) technology. According to clinicopathological diagnosis, these patients were grouped in a training set (35 EoE, 30 RE, 30 NH) for building of a three-class prediction model using the random forest method, and a blinded predictive set (n = 47) for model validation. A diagnostic model built on ten differentially expressed genes was able to differentiate with 100% sensitivity and specificity between conditions in the training set. In a blinded predictive set, this model was able to correctly predict EoE in 14 of 18 patients in distal (sensitivity 78%,...Continue Reading

References

Feb 3, 2006·The Journal of Clinical Investigation·Carine BlanchardMarc E Rothenberg
Sep 22, 2007·Current Opinion in Pediatrics·Terri Brown-Whitehorn, Chris A Liacouras
Feb 19, 2008·Nature Biotechnology·Gary K GeissKrassen Dimitrov
Feb 12, 2009·The American Journal of Gastroenterology·Ameesh ShahIkuo Hirano
Jul 15, 2009·Gastroenterology·Marc E Rothenberg
Jun 12, 2010·The Journal of Allergy and Clinical Immunology·J Pablo AboniaMarc E Rothenberg
Jan 8, 2011·The Journal of Allergy and Clinical Immunology·Carine BlanchardMarc E Rothenberg
Mar 25, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A M SchoepferA Straumann
Apr 12, 2011·The Journal of Allergy and Clinical Immunology·Chris A LiacourasSeema S Aceves
Oct 25, 2011·The Journal of Allergy and Clinical Immunology·Petr HruzUNKNOWN Swiss EoE study group
Jul 31, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W S LexmondE Fiebiger
Aug 28, 2013·Gastroenterology·Ting WenMarc E Rothenberg
Jan 1, 2014·Journal of Pediatric Gastroenterology and Nutrition·A PapadopoulouY Vandenplas
Jan 22, 2014·The American Journal of Gastroenterology·Jennifer A NielsenCory A Roberts
Mar 8, 2014·Digestive Diseases·David A Katzka

❮ Previous
Next ❯

Citations

Feb 10, 2017·Clinical and Translational Gastroenterology·Evan S DellonRichard H Lash
Nov 21, 2018·Frontiers in Immunology·Benjamin F SallisEdda Fiebiger
Sep 30, 2015·Current Opinion in Pediatrics·Michiko K Oyoshi
Mar 25, 2017·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Dan AtkinsJonathan M Spergel
Sep 30, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G RobertsJ A Woodfolk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.