Accuracy of preimplantation genetic diagnosis in equine in vivo-recovered and in vitro-produced blastocysts

Reproduction, Fertility, and Development
Y H ChoiK Hinrichs

Abstract

Preimplantation genetic diagnosis has great potential in the horse, but information on evaluation of equine embryo biopsy samples is limited. Blastocysts were biopsied using a Piezo drill and methods for whole-genome amplification (WGA) investigated. Results for 33 genetic loci were then compared between biopsy samples from in vitro-produced (IVP) and in vivo-recovered (VIV) blastocysts. Under the experimental conditions described, WGA using the Qiagen Repli-g Midi kit was more accurate than that using the Illustra Genomiphi V2 kit (98.2% vs 25.8%, respectively). Using WGA with the Qiagen kit, three biopsy samples were evaluated from each of eight IVP and 19 VIV blastocysts, some produced using semen from stallions carrying the genetic mutations associated with the diseases hereditary equine regional dermal asthenia (HERDA), hyperkalemic periodic paralysis (HYPP) or polysaccharide storage myopathy 1 (PSSM1). Three of 81 biopsy samples (3.7%) returned 95% overall accuracy in IVP and VIV embryos, and this technique is suitable for use in a clinical setting.

References

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Sep 17, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Y H ChoiK Hinrichs
Apr 5, 2011·Theriogenology·Y H ChoiK Hinrichs

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Citations

Apr 15, 2018·Equine Veterinary Journal·L H A Morris
Sep 22, 2018·Reproduction in Domestic Animals = Zuchthygiene·Katrin Hinrichs
Nov 16, 2019·Reproduction, Fertility, and Development·E L Squires
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Achraf BenammarPascale Chavatte-Palmer

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Methods Mentioned

BETA
biopsy
PCR
biopsies
electrophoresis
genotyping
biospies

Software Mentioned

SigmaPlot
Epistat Statistical Package
Illustra Genomiphi
STRand

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