Accuracy of sampling methods in morphometric studies of human sural nerves

Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia
Z CaiP C Blumbergs

Abstract

The aim of this study was to ascertain the minimum sample required to accurately measure the total number of myelinated fibres, mean myelinated fibre density (MFD), myelinated fibre diameter (Ds) and axonal diameter (Da) in morphometric studies of sural nerve biopsies. Measurements were obtained by sampling a single fascicle or systematic sampling of up to 50% of the total transverse fascicular area of two control and eighteen pathological sural nerves showing varying degrees of demyelination and axonal degeneration. MFD and fibre size were heterogeneous between fascicles in both control and pathological sural nerves, and morphometric results from one fascicle and systematic sampling of up to 50% of the total transverse fascicular area did not accurately represent the whole myelinated fibre population in the sural nerve. For accurate morphometric data it is necessary to quantitate all the myelinated fibres in the sural nerve.

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Citations

Jul 11, 2013·BioMed Research International·Antônio Paulo da Costa Bilego NetoValéria Paula Sassoli Fazan
May 9, 2006·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Z CaiP D Thompson
Apr 11, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Michael B Christensen, Patrick A Tresco
Feb 4, 2010·Anatomical Science International·Vilai ChentanezAthitaya Kaewsema
Jun 23, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John FiebergJacqueline L Frair
Feb 1, 2017·Death Studies·Annika Jonsson, Tony Walter
May 3, 2021·Maxillofacial Plastic and Reconstructive Surgery·Soo-Hwan Byun, Kang-Min Ahn

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