Accuracy of three automated systems (MicroScan WalkAway, VITEK, and VITEK 2) for susceptibility testing of Pseudomonas aeruginosa against five broad-spectrum beta-lactam agents

Journal of Clinical Microbiology
Hélio S SaderRonald N Jones

Abstract

One hundred recent clinical Pseudomonas aeruginosa isolates were used to assess the quantitative (MIC) and qualitative (susceptibility category) accuracies of the MicroScan WalkAway, VITEK, and VITEK 2 automated susceptibility test systems when five-broad spectrum beta-lactams, aztreonam, cefepime, ceftazidime, imipenem, and piperacillin-tazobactam, were tested. Isolates were selected so that the MICs for the isolates overrepresented the MICs near the breakpoints to assess precisely the agreement between the results obtained with the automated systems and the results obtained by the reference tests. The categorical and MIC results from the automated systems were compared to the consensus result of three reference methods: broth microdilution, agar dilution, and disk diffusion. The consensus categorical testing (susceptibility and resistance) rates were 47 and 27%, respectively, for aztreonam; 59 and 14%, respectively, for cefepime; 44 and 43%, respectively, for ceftazidime; 71 and 19%, respectively, for imipenem; and 50 and 50%, respectively, for piperacillin-tazobactam. All systems tested exhibited a high, unacceptable level of very major (false-susceptible) errors for piperacillin-tazobactam (19 to 27%). Major (false-resistan...Continue Reading

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Citations

Jun 23, 2010·Seminars in Plastic Surgery·Jason H CalhounMark Shirtliff
Jul 20, 2010·Diagnostic Microbiology and Infectious Disease·Dong Wook JekarlYeon-Joon Park
May 1, 2007·Diagnostic Microbiology and Infectious Disease·Thean Yen TanKaren Poh
Oct 28, 2009·Pharmacotherapy·Kristi M KuperAudrey Wanger
Jul 20, 2011·Diagnostic Microbiology and Infectious Disease·Won-Young JinYoung-Jin Park
Feb 3, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N MesarosF Van Bambeke
Dec 19, 2014·Science Translational Medicine·Jungil ChoiSunghoon Kwon
Jan 11, 2017·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Marguerite L MonogueDavid P Nicolau
Nov 20, 2009·Scandinavian Journal of Infectious Diseases·Toshinobu HoriiMotoki Morita
Nov 17, 2006·Journal of Medical Microbiology·J R W Govan
Nov 21, 2020·International Journal of Molecular Sciences·Minh Tam Tran ThiBernd H A Rehm
Sep 29, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Thomas GehringChristian Buechler

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