Accuracy of three diagnostic tests for determining Mycobacterium bovis infection status in live-sampled wild meerkats (Suricata suricatta)

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
J A DreweG P Pearce

Abstract

Accurate diagnosis of Mycobacterium bovis infection (bovine tuberculosis [bTB]) in live animals is notoriously problematic. The aim of the present study was to evaluate the diagnostic potential of 2 new serologic tests (multiantigen print immunoassay [MAPIA] and lateral flow immunoassay rapid test [RT]) in comparison with mycobacterial culture of tracheal washes for determining M. bovis infection status in a free-ranging population of wild meerkats (Suricata suricatta). During a longitudinal study lasting 2.5 years, 240 individually identifiable meerkats were each sampled up to 8 times under anesthesia every 3 months. Diagnostic accuracy was determined through Bayesian and maximum likelihood estimations of sensitivity, specificity, and likelihood ratios for each diagnostic test when used independently and in parallel to classify the disease status of individual meerkats in the absence of a gold standard. Culture of tracheal washes was highly specific (0.99; 95% confidence interval [CI] = 0.77, 1.00) but of low sensitivity (0.36; 95% CI = 0.24, 0.50) for diagnosing M. bovis-infected individuals. The longitudinal nature of the study with repeated sampling of the same individual animals served simultaneously to improve chances of ...Continue Reading

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Citations

Nov 6, 2009·Proceedings. Biological Sciences·Julian A Drewe
Mar 6, 2013·PLoS Neglected Tropical Diseases·Sonja HartnackPaul R Torgerson
Jul 26, 2011·Journal of Comparative Pathology·C J PalgraveN H Smith
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Jun 2, 2016·Journal of the South African Veterinary Association·Charlene ClarkeSven Parsons
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Sep 3, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Karla A FentonKonstantin P Lyashchenko
May 30, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Beibei JiaIan A Gardner
Feb 16, 2019·Ecology and Evolution·Graziella V DiRenzoElise F Zipkin
Nov 14, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Elisabeth M D L van der HeijdenAnita L Michel
Oct 22, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ismari Perini FurlanetoKarla Valéria Batista Lima
Feb 26, 2021·Veterinary Research·Jobin ThomasMaría A Risalde
Nov 1, 2016·Journal of the South African Veterinary Association·Charlene ClarkeSven Parsons

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

R
MAPIA
WinBUGS
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