Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification.

Analytical and Bioanalytical Chemistry
Lieve M L LaurensEdward J Wolfrum

Abstract

In the context of algal biofuels, lipids, or better aliphatic chains of the fatty acids, are perhaps the most important constituents of algal biomass. Accurate quantification of lipids and their respective fuel yield is crucial for comparison of algal strains and growth conditions and for process monitoring. As an alternative to traditional solvent-based lipid extraction procedures, we have developed a robust whole-biomass in situ transesterification procedure for quantification of algal lipids (as fatty acid methyl esters, FAMEs) that (a) can be carried out on a small scale (using 4-7 mg of biomass), (b) is applicable to a range of different species, (c) consists of a single-step reaction, (d) is robust over a range of different temperature and time combinations, and (e) tolerant to at least 50% water in the biomass. Unlike gravimetric lipid quantification, which can over- or underestimate the lipid content, whole biomass transesterification reflects the true potential fuel yield of algal biomass. We report here on the comparison of the yield of FAMEs by using different catalysts and catalyst combinations, with the acid catalyst HCl providing a consistently high level of conversion of fatty acids with a precision of 1.9% relat...Continue Reading

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Citations

Feb 5, 2014·Journal of Bioscience and Bioengineering·Peng ZhaoZunxi Huang
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Apr 22, 2017·World Journal of Microbiology & Biotechnology·Ryosuke YamadaHiroyasu Ogino
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Mar 13, 2021·Biotechnology for Biofuels·Gonzalo M Figueroa-TorresConstantinos Theodoropoulos

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