Accurate differentiation of Mycobacterium chimaera from Mycobacterium intracellulare by MALDI-TOF MS analysis

Journal of Medical Microbiology
Arthur B PranadaMarkus Timke

Abstract

The increasing number of infections caused by nontuberculous mycobacteria (NTM) has prompted the need for rapid and precise identification methods of these pathogens. Several studies report the applicability of MALDI-TOF mass spectrometry (MS) for identification of NTM. However, some closely related species have very similar spectral mass fingerprints, and until recently, Mycobacterium chimaera and M. intracellulare could not be separated from each other by MALDI-TOF MS. The conventional identification methods used in routine diagnostics have similar limitations. Recently, the differentiation of these two species within the Mycobacterium avium complex has become increasingly important due to reports of M. chimaera infections related to open heart surgery in Europe and in the USA. In this report, a method for the distinct differentiation of M. chimaera and M. intracellulare using a more detailed analysis of MALDI-TOF mass spectra is presented. Species-specific peaks could be identified and it was possible to assign all isolates (100 %) from reference strain collections as well as clinical isolates to the correct species. We have developed a model for the accurate identification of M. chimaera and M. intracellulare by MALDI-TOF M...Continue Reading

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Citations

Sep 21, 2018·International Journal of Systematic and Evolutionary Microbiology·Jakko van IngenBarbara A Brown-Elliott
Oct 30, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon H Kasperbauer, Charles L Daley
Jul 16, 2019·Expert Review of Molecular Diagnostics·Markus KostrzewaArthur B Pranada
Jul 18, 2019·Expert Review of Proteomics·Martin WelkerDavid Pincus
Apr 17, 2019·Journal of Food and Drug Analysis·Tsung-Yun HouShih-Hua Teng
Jul 29, 2020·Journal of Clinical Microbiology·David Rodriguez-TemporalFernando Alcaide
Feb 8, 2018·Expert Review of Proteomics·Markus Kostrzewa
Jan 31, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Belén Rodríguez-SánchezGilbert Greub
Mar 21, 2018·Current Infectious Disease Reports·Elizabeth Ann MischJames Muse Davis
Dec 14, 2018·Frontiers in Microbiology·Miriam CordovanaArthur B Pranada
May 22, 2020·Current Dermatology Reports·Samuel YeroushalmiAdam Friedman
Apr 30, 2020·Enfermedades infecciosas y microbiología clínica·Marina Oviaño, Belén Rodríguez-Sánchez
Jul 17, 2021·Enfermedades infecciosas y microbiología clínica·Ramiro López MedranoOctavio Miguel Rivero Lezcano
Feb 15, 2019·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Suhail Ahmad, Eiman Mokaddas

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