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Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation

Biophysical Journal

Jan 18, 2005

Nam Ki LeeShimon Weiss

Abstract

Fluorescence resonance energy transfer (FRET) between a donor (D) and an acceptor (A) at the single-molecule level currently provides qualitative information about distance, and quantitative information about kinetics of distance changes. Here, we used the sorting ability of confocal mi...read more

Mentioned in this Paper

Molecular Helix
Glycerin
Derivatives
Alkalescens-Dispar Group
Cross-talk
Nucleic Acid Hybridization Procedure
Extravasation
Complex (molecular entity)
Transcription, Genetic
Helix (Snails)
359
Paper Details
References
  • References31
  • Citations133
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  • References31
  • Citations133
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Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation

Biophysical Journal

Jan 18, 2005

Nam Ki LeeShimon Weiss

PMID: 15653725

DOI: 10.1529/biophysj.104.054114

Abstract

Fluorescence resonance energy transfer (FRET) between a donor (D) and an acceptor (A) at the single-molecule level currently provides qualitative information about distance, and quantitative information about kinetics of distance changes. Here, we used the sorting ability of confocal mi...read more

Mentioned in this Paper

Molecular Helix
Glycerin
Derivatives
Alkalescens-Dispar Group
Cross-talk
Nucleic Acid Hybridization Procedure
Extravasation
Complex (molecular entity)
Transcription, Genetic
Helix (Snails)
359

Feeds With Similar Papers

Cytoplasmic Biophysics

Cytoplasmic biophysics is an interdisciplinary science that applies approaches and methods traditionally used in physics to study the biological structure and function of cytoplasm which consists of cytosol and organelles. Discover the latest research on cytoplasmic biophysics here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules

Proceedings of the National Academy of Sciences of the United States of AmericaJune 4, 2004
Achillefs N KapanidisShimon Weiss
Proceedings of the National Academy of Sciences of the United States of America

Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Förster distance dependence and subpopulations

Proceedings of the National Academy of Sciences of the United States of AmericaMarch 31, 1999
A A DenizP G Schultz
Paper Details
References
  • References31
  • Citations133
1234
  • References31
  • Citations133
12345...

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