Accurate mass measurement of synthetic analogues of prazosine by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
C AndalòG C Galletti

Abstract

Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) has been investigated as a tool for accurate determination of the molecular mass of synthetic analogues of prazosine, a molecule used for the treatment of hypertension. Samples were dissolved in methanol, mixed with mass calibration standards, and crystallised on the target with alpha-cyano-4-hydroxycinnamic acid as matrix. Acquisition of spectra was rapidly completed in reflectron mode, allowing high resolution (6000-10000) and sensitive (about 1-10 pmol of sample on target) determination of the synthetic products. The results show the effectiveness of MALDI-TOFMS for accurate mass determination of these fairly large molecules, which are otherwise difficult to analyse by other high-resolution mass spectrometric techniques.

References

Jun 1, 1994·Trends in Pharmacological Sciences·A P FordD E Clarke
Apr 25, 1997·Journal of Medicinal Chemistry·B KennyD Fox
Nov 14, 1997·Medicinal Research Reviews·P Mátyus, K Horváth

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Citations

Oct 27, 2009·Journal of the American Society for Mass Spectrometry·Andrew Kruegel, Athula B Attygalle
Aug 28, 2001·Journal of Mass Spectrometry : JMS

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