PMID: 20632944Jul 17, 2010Paper

Accurate mass tag retention time database for urine proteome analysis by chromatography--mass spectrometry

Biochemistry. Biokhimii︠a︡
I A AgronE N Nikolaev

Abstract

Information about peptides and proteins in urine can be used to search for biomarkers of early stages of various diseases. The main technology currently used for identification of peptides and proteins is tandem mass spectrometry, in which peptides are identified by mass spectra of their fragmentation products. However, the presence of the fragmentation stage decreases sensitivity of analysis and increases its duration. We have developed a method for identification of human urinary proteins and peptides. This method based on the accurate mass and time tag (AMT) method does not use tandem mass spectrometry. The database of AMT tags containing more than 1381 AMT tags of peptides has been constructed. The software for database filling with AMT tags, normalizing the chromatograms, database application for identification of proteins and peptides, and their quantitative estimation has been developed. The new procedures for peptide identification by tandem mass spectra and the AMT tag database are proposed. The paper also lists novel proteins that have been identified in human urine for the first time.

References

Aug 12, 2000·Analytical Chemistry·T P ConradsR D Smith
Feb 22, 2002·Journal of Mass Spectrometry : JMS·Marco Wehofsky, Ralf Hoffmann
Jun 1, 1993·Current Biology : CB·D J PappinA J Bleasby
Sep 25, 2004·Mass Spectrometry Reviews·Bogdan Bogdanov, Richard D Smith

❮ Previous
Next ❯

Citations

Dec 10, 2013·Bulletin of Experimental Biology and Medicine·L Kh PastushkovaK S Kireev
Aug 24, 2013·PloS One·Lyudmila Kh PastushkovaEvgeny N Nikolaev
Nov 14, 2013·International Journal of Proteomics·Holger HusiJames A Ross
Jun 18, 2011·Expert Review of Proteomics·Juan Casado-VelaJuan Carlos Lacal
Nov 4, 2010·Advances in Chronic Kidney Disease·Michael L Merchant
Jan 13, 2015·Journal of Bioinformatics and Computational Biology·Irina M LarinaVladimir A Ivanisenko
Jan 8, 2015·BMC Genomics·Hans BinderIrina M Larina
Oct 26, 2016·Journal of Proteomics·A S KononikhinG T Sukhikh
Oct 26, 2016·Journal of Proteomics·Natalia L StarodubtsevaGennady T Sukhikh
Jan 1, 2014·European Journal of Mass Spectrometry·Vladimir G Zaikin, Alexander A Sysoev
Jan 8, 2013·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V I FedchenkoA E Medvedev
Mar 18, 2016·International Journal of Oncology·Holger HusiJames A Ross

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Marianne SchmidMichael Joannidis
Journal of Proteome Research
Visith Thongboonkerd
Journal of Chromatography. a
Yasushi IshihamaMatthias Mann
© 2021 Meta ULC. All rights reserved