Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

Journal of Magnetic Resonance
Katalin E KövérKrisztina Fehér

Abstract

Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite pi pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.

References

Sep 5, 2001·Journal of Magnetic Resonance·M D Hürlimann
Aug 25, 2004·Journal of Magnetic Resonance·H KoskelaS Heikkinen
Sep 1, 1996·Journal of Biomolecular NMR·F A MulderR Boelens

❮ Previous
Next ❯

Citations

Jul 9, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christina BraunbergerLiselotte Krenn
Aug 22, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Teodor Parella, Juan Félix Espinosa
Oct 27, 2009·Journal of Magnetic Resonance·Harri KoskelaSami Heikkinen
Jun 10, 2008·Journal of Magnetic Resonance·J W WienchM Pruski
Apr 16, 2008·Journal of Magnetic Resonance·Andreas EnthartBurkhard Luy
May 18, 2016·Journal of Magnetic Resonance·Simon GlanzerKlaus Zangger
Jan 18, 2018·Magnetic Resonance in Chemistry : MRC·Peter Bigler, Julien Furrer
Jun 17, 2018·Magnetic Resonance in Chemistry : MRC·Peter Bigler, Julien Furrer
Sep 15, 2006·Magnetic Resonance in Chemistry : MRC·Pau NolisTeodor Parella
Dec 1, 2018·Magnetic Resonance in Chemistry : MRC·Peter Bigler, Julien Furrer
Jan 9, 2008·Magnetic Resonance in Chemistry : MRC·Susimaire PedersoliCláudio F Tormena
Mar 5, 2019·Chembiochem : a European Journal of Chemical Biology·Mária RaicsKatalin E Kövér
Jul 14, 2011·Magnetic Resonance in Chemistry : MRC·Juan F EspinosaSergi Gil
Jan 20, 2011·Magnetic Resonance in Chemistry : MRC·Sándor Boros, Katalin E Kövér
Jun 20, 2013·Magnetic Resonance in Chemistry : MRC·Josep Saurí, Teodor Parella
Mar 10, 2011·Magnetic Resonance in Chemistry : MRC·Katalin E KövérLászló Szilágyi
Jun 26, 2014·Angewandte Chemie·Laura CastañarTeodor Parella
May 30, 2013·Organic & Biomolecular Chemistry·Josep SauríJuan F Espinosa
Dec 15, 2017·Magnetic Resonance in Chemistry : MRC·István Timári, Katalin E Kövér
Feb 15, 2008·Journal of Medicinal Chemistry·Máté ErdélyiTeresa Carlomagno
Nov 9, 2011·The Journal of Physical Chemistry. a·Denize C FavaroCláudio F Tormena

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.