Accurate photodensitometric measurement of the lecithin: sphingomyelin ration after elimination of the acidic phospholipids from extracts of amniotic fluid specimens

Clinica Chimica Acta; International Journal of Clinical Chemistry
L Gosselin, J M Foidart


Treatment of human amniotic fluid lipids dissolved in a chloroform/methanol (9:1, v/v) mixture by batchwise addition of diethylaminoethyl cellulose in a dry state proved to be an easy and rapid procedure for the removal of the acidic phospholipids which may interfere in the photodensitometric evaluation of the lecithin: spingomyelin ratio on a thin-layer chromatogram. This method was used for the measurement of the lecithin: sphingomyelin ratio in a series of normal and abnormal pregnancies. Ratios higher than expected from gestational age were observed in stressed pregnancies. A significant elevation of the ratio was also observed under treatment by dexamethasone.


Jul 10, 2003·Clinics in Laboratory Medicine·J S Torday, V K Rehan
Jan 1, 1982·Critical Reviews in Clinical Laboratory Sciences·L M Brown, C G Duck-Chong
Jul 1, 1979·Annals of Clinical Biochemistry·C G Duck-Chong, L M Brown

Related Concepts

Amniotic Fluid Index
Thin Layer Chromatography
Gestational Age
Study, Methodological

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