Accurate quantification of fourteen normal bone marrow cell subsets in infants to the elderly by flow cytometry

Cytometry. Part B, Clinical Cytometry
Julie PontMarie-Christine Jacob

Abstract

Studies of normal bone marrow (BM) cell composition by flow cytometry are scarce. Presently, we aimed to quantify 14 cell subsets from infants to elderly patients. Cell subsets in BM samples from 180 individuals without morphologically abnormal leukocytes were analyzed using a single combination of eight antibodies: CD3/CD10/CD38/CD19/CD36/CD16/CD34/CD45. By comparison with the Holdrinet score, we first validated the immature granulocyte/neutrophil (IGRA/N) ratio as a readily obtainable criterion of BM sample purity in 145 cases. Then, the 115 highly pure samples were selected (IGRA/N ≥ 1.2) and analyzed according to age group. CD34+ myeloblasts became progressively more infrequent with age: median 1.4% in infancy to 0.5% in the elderly. Neutrophils increased: 10.7% to 22.8%; all other myeloid subsets, IGRA, eosinophils, basophils and monocytes remained stable: respectively 40.3% to 46.7%, 2.0% to 2.8%, 0.2% to 0.3%, and 4.4% to 5.0% throughout life. Erythroblasts were lower in children (8.4% to 10.3%) than in adults (12.5% to 15.1%). For lymphoid cells, hematogones and transitional B-cells decreased: 15.5% to 0.6% and 3.6% to 0.1%, respectively; mature lymphocytes remained stable: B-cells: 1.4% to 2.8%, T-cells: 5.8% to 8.7%, ...Continue Reading

References

Apr 12, 2002·Journal of Clinical Laboratory Analysis·Ryousuke YamamuraNoriyuki Tatsumi
Jan 15, 2003·Journal of Clinical Laboratory Analysis·Hisako ShibataMasayuki Hino
Jun 13, 2008·Cytometry. Part B, Clinical Cytometry·Michael R LokenDenise A Wells
Sep 6, 2012·Leukemia·T KalinaUNKNOWN EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Oct 24, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Françoise SollyMarie C Béné
Mar 19, 2016·Cytometry. Part B, Clinical Cytometry·Marie-Christine JacobJean-Yves Cesbron

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Citations

Nov 19, 2019·Cytometry. Part B, Clinical Cytometry·Francis Mandy
May 26, 2020·International Journal of Laboratory Hematology·Georgina GenerMarc Sorigue
Dec 12, 2021·Cytometry. Part B, Clinical Cytometry·Vincent H J van der VeldenArjan van de Loosdrecht
Dec 22, 2021·Cytometry. Part B, Clinical Cytometry·Ulrika JohanssonTheresia M Westers

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