Accurate quantification of T-cells expressing PD-1 in patients on anti-PD-1 immunotherapy

Cancer Immunology, Immunotherapy : CII
Henning ZelbaBenjamin Weide

Abstract

Increasing numbers of trials employing anti-PD-1 immunotherapy emphasize the requirement for predictive biomarkers of clinical response. Many studies examine the cell surface expression of PD-1 and other key regulators of T-cell activation and inhibition. Here, we compared common commercially available anti-PD-1 diagnostic antibodies and tested whether they can bind the PD-1 receptor in the presence of the therapeutic antagonists pembrolizumab and nivolumab. We observed that currently no antibodies are available that can reliably stain all PD-1 receptors on T-cells from patients treated with anti-PD-1 antibodies. Furthermore, none of the diagnostic antibodies detected the entire population of PD-1+ T-cells relative to indirect staining using the therapeutic antibodies themselves. To overcome this problem, here we present a reliable method for quantifying PD-1 expression on immune cells from treated patients which can be included in any conventional flow or mass cytometry antibody panel used for patient monitoring.

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Citations

Mar 13, 2019·Journal of Experimental & Clinical Cancer Research : CR·Linan FangLi Zhang
Jun 26, 2020·Frontiers in Immunology·Kilian Wistuba-HamprechtGraham Pawelec
Jun 14, 2019·Frontiers in Medicine·Moira GravesNikola A Bowden
Jan 6, 2021·Cancer Immunology, Immunotherapy : CII·Alexander W MacFarlaneNamrata Vijayvergia
Feb 20, 2021·HemaSphere·Jan Christoph BanckMichael von Bergwelt-Baildon
Mar 12, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Fabian JunkerHenry Kao
Apr 13, 2021·Frontiers in Oncology·Vinicius Araujo B de LimaInge Marie Svane

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