Accurate single-molecule FRET studies using multiparameter fluorescence detection

Methods in Enzymology
Evangelos SisamakisClaus A M Seidel

Abstract

In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing important insight into how biological molecules function. So far our view of protein function is formed, to a significant extent, by traditional structure determination showing many beautiful static protein structures. Recent experiments by single-molecule and other techniques have questioned the idea that proteins and other biomolecules are static structures. In particular, Förster resonance energy transfer (FRET) studies of single molecules have shown that biomolecules may adopt many conformations as they perform their function. Despite the success of sm-studies, interpretation of smFRET data are challenging since they can be complicated due to many artifacts arising from the complex photophysical behavior of fluorophores, dynamics, and motion of fluorophores, as well as from small amounts of contaminants. We demonstrate that the simultaneous acquisition of a maximum of fluorescence parameters by multiparameter fluorescence detection (MFD) allows for a robust assessment of all possible artifacts arising from smFRET and offers unsurpassed capabilities regarding the identification and analysis of individual species present in a population o...Continue Reading

Citations

Mar 23, 2011·The Journal of Physical Chemistry. a·Amy L SpeelmanBrent P Krueger
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·James J McCannMark E Bowen
May 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Irina V Gopich, Attila Szabo
Jan 17, 2012·PloS One·David KellyKaroline Wiesner
Dec 4, 2012·International Journal of Molecular Sciences·Jörg Enderlein
Oct 5, 2013·The Journal of Chemical Physics·Hagen HofmannBenjamin Schuler
Jan 12, 2011·Annual Review of Physical Chemistry·Michelle A Digman, Enrico Gratton
Jan 10, 2016·The Journal of Physical Chemistry. B·Florian StockmarGerd Ulrich Nienhaus
Dec 15, 2015·Journal of the American Chemical Society·Laura E Baltierra-JassoSteven W Magennis
Oct 2, 2015·Journal of the American Chemical Society·Elise DelaforgeMartin Blackledge
Jan 24, 2015·The Journal of Chemical Physics·Irina V Gopich
May 6, 2016·Annual Review of Biophysics·Benjamin SchulerDaniel Nettels
Oct 21, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Charlotte E DaltonSteven W Magennis
Nov 28, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Henning HöfigJörg Fitter
Jan 22, 2017·Analytical Biochemistry·Alexander KyrychenkoAlexey S Ladokhin
Mar 16, 2017·Biophysical Journal·Marijonas TutkusDanielis Rutkauskas
Sep 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andreas HartmannMichael Schlierf
Oct 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexander GustDina Grohmann
Sep 1, 2012·Chembiochem : a European Journal of Chemical Biology·Søren Preus, L Marcus Wilhelmsson
Jan 18, 2018·Nature Communications·Sinan KilicBeat Fierz
Feb 22, 2018·Nature·Alessandro BorgiaBenjamin Schuler
Feb 24, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Tobias RosenkranzJörg Fitter
Dec 12, 2012·Nature Methods·Timothy D Craggs, Achillefs N Kapanidis
Nov 13, 2012·Nature Methods·Stanislav KalininClaus A M Seidel
Nov 1, 2016·Nature Methods·Christine KoehlerEdward A Lemke
Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·X MichaletS Cova
Jan 10, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Laura E Baltierra-JassoSteven W Magennis
Feb 26, 2020·Annual Review of Physical Chemistry·Lauren Ann Metskas, Elizabeth Rhoades
Feb 6, 2015·Nature Communications·Stephan BenkeBenjamin Schuler

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