ACE-inhibition prevents postischemic coronary leukocyte adhesion and leukocyte-dependent reperfusion injury

Cardiovascular Research
C KupattE Gerlach

Abstract

Polymorphonuclear leukocytes (PMN), retained in the microvascular bed, can contribute to postischemic myocardial reperfusion injury. Since a beneficial effect of ACE-inhibition on reperfusion injury has been reported, we investigated the impact of cilazaprilat on PMN dependent reperfusion injury in isolated guinea pig hearts. Hearts (n = 5 per group) were subjected to 15 min of ischemia. Immediately thereafter, a bolus of PMN was injected into the coronary system. External heart work (EHW) and total cardiac nitric oxide release were measured. For microscopic evaluation, hearts received rhodamine 6G labelled PMN after ischemia, were arrested 5 min later and further perfused with FITC dextran (0.1%). Localization of retained PMN was assessed by fluorescence microscopy. Leukocyte activation was studied by FACS analysis of the adhesion molecule CD11b before and after coronary passage of the PMN. The ACE-inhibitor cilazaprilat (Cila, 2 microM) and the NO-synthase inhibitor nitro-L-arginine (NOLAG, 10 microM) were used to modulate nitric oxide formation of the heart. Postischemic EHW recovered to 67 +/- 5% (controls) and 64 +/- 6% (Cila) of the preischemic value. Addition of PMN severely depressed recovery of EHW (39 +/- 2%) and NO r...Continue Reading

Citations

Sep 23, 2010·Basic Research in Cardiology·Bernhard F BeckerMatthias Jacob
Jan 5, 2002·European Journal of Pharmacology·Michał KurzelewskiAndrzej Beresewicz
Apr 5, 2003·The International Journal of Biochemistry & Cell Biology·Yusuke SuzukiJesus Egido
May 14, 2010·Cardiovascular Research·Bernhard F BeckerMatthias Jacob
Jun 30, 2007·The Journal of Thoracic and Cardiovascular Surgery·René SchrammHans-Joachim Schäfers
Jan 8, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Yu ChenJames P Morgan
Oct 4, 2005·Nature Reviews. Immunology·Ashley T Haase
Feb 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian KupattPeter Boekstegers
Oct 4, 2005·Nature Reviews. Immunology·Marko Salmi, Sirpa Jalkanen
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick G ArndtG Scott Worthen
Dec 19, 2019·Frontiers in Immunology·Dean Philip John Kavanagh, Neena Kalia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.