ACE2 receptor expression and severe acute respiratory syndrome coronavirus infection depend on differentiation of human airway epithelia

Journal of Virology
Hong Peng JiaPaul B McCray

Abstract

Studies of patients with severe acute respiratory syndrome (SARS) demonstrate that the respiratory tract is a major site of SARS-coronavirus (CoV) infection and disease morbidity. We studied host-pathogen interactions using native lung tissue and a model of well-differentiated cultures of primary human airway epithelia. Angiotensin converting enzyme 2 (ACE2), the receptor for both the SARS-CoV and the related human respiratory coronavirus NL63, was expressed in human airway epithelia as well as lung parenchyma. As assessed by immunofluorescence staining and membrane biotinylation, ACE2 protein was more abundantly expressed on the apical than the basolateral surface of polarized airway epithelia. Interestingly, ACE2 expression positively correlated with the differentiation state of epithelia. Undifferentiated cells expressing little ACE2 were poorly infected with SARS-CoV, while well-differentiated cells expressing more ACE2 were readily infected. Expression of ACE2 in poorly differentiated epithelia facilitated SARS spike (S) protein-pseudotyped virus entry. Consistent with the expression pattern of ACE2, the entry of SARS-CoV or a lentivirus pseudotyped with SARS-CoV S protein in differentiated epithelia was more efficient whe...Continue Reading

Associated Clinical Trials

References

Nov 1, 1974·The Journal of Infectious Diseases·K McIntoshM A Mufson
Sep 23, 1967·British Medical Journal·A F BradburneD A Tyrrell
Nov 24, 1999·The Journal of Clinical Investigation·L MazzucchelliM Uguccioni
Jun 29, 2000·Gene Therapy·R D AndersonB L Davidson
Jun 29, 2000·American Journal of Respiratory Cell and Molecular Biology·S L BrodyS D Shapiro
Jul 8, 2000·Circulation Research·P J Pagano
Feb 7, 2002·The European Respiratory Journal·M A ChilversC O'Callaghan
May 4, 2002·Methods in Molecular Biology·Philip H KarpMichael J Welsh
Apr 3, 2003·The New England Journal of Medicine·Susan M PoutanenUNKNOWN Canadian Severe Acute Respiratory Syndrome Study Team
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
May 6, 2003·Science·Marco A MarraRachel L Roper
May 16, 2003·Nature·Ron A M FouchierAlbert D M E Osterhaus
Jun 5, 2003·Lancet·John M NichollsJ S Malik Peiris
Oct 31, 2003·Nature·Byron E E MartinaAlbert D M E Osterhaus
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates
Mar 23, 2004·Nature Medicine·Lia van der HoekBen Berkhout
Apr 23, 2004·The New England Journal of Medicine·Ignatius T S YuTommy Ho
May 29, 2004·Trends in Pharmacological Sciences·Anthony J TurnerNigel M Hooper
Jul 24, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·David M HwangJagdish Butany
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·William G GlassPhilip M Murphy
Sep 11, 2004·Biochemical and Biophysical Research Communications·Yuchun NieHongkui Deng
Sep 29, 2004·Journal of Virology·Thomas RoweJames M Wilson
Oct 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Scott A JeffersKathryn V Holmes
Dec 15, 2004·Journal of Virology·Anjeanette RobertsKanta Subbarao
Mar 26, 2005·The EMBO Journal·Wenhui LiMichael Farzan
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Heike HofmannStefan Pöhlmann

❮ Previous
Next ❯

Citations

Jan 25, 2007·Annual Review of Immunology·Jun Chen, Kanta Subbarao
May 5, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hong Peng JiaPaul B McCray
Jul 30, 2009·Journal of Leukocyte Biology·Tanya A Miura, Kathryn V Holmes
Feb 19, 2013·American Journal of Respiratory Cell and Molecular Biology·Zhaohui QianRobert J Mason
Jan 24, 2016·Journal of Biomedical Science·Yvonne Ting-Chun YuMing-Fu Chang
Mar 30, 2007·The American Journal of Pathology·Jiang Gu, Christine Korteweg
Apr 17, 2014·Reviews in Medical Virology·Yingying Cong, Xiaofeng Ren
Oct 9, 2014·The Journal of Pathology·Judith Ma van den BrandBart L Haagmans
Aug 9, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Joshua M OakesXinping Yue
Mar 7, 2007·Journal of Cellular Biochemistry·Renda Soylemez WienerMary C Williams
Apr 28, 2007·The Journal of Pathology·I HammingH van Goor
Apr 7, 2006·Expert Review of Anti-infective Therapy·Erik De Clercq
Feb 6, 2020·Journal of Travel Medicine·Mary E Wilson, Lin H Chen
Apr 16, 2020·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Vanesa PalauMaría José Soler
May 21, 2020·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Spoorthy KulkarniIan Wilkinson
May 21, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kimberly E StelzigSergio E Chiarella
May 23, 2020·Journal of the American Heart Association·Tarek AlsaiedArwa Saidi
Apr 30, 2020·Nature Reviews. Immunology·Matthew Zirui TayLisa F P Ng
Jun 6, 2020·Pharmacogenomics·Eric M Snyder, Bruce D Johnson
May 15, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Rachel BushKiran Upadhyay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.