PMID: 15375576Sep 18, 2004Paper

Acellular liver matrix improves the survival and functions of isolated rat hepatocytes cultured in vitro

International Journal of Molecular Medicine
Patrizia BurraGastone G Nussdorfer

Abstract

Alternative approaches to overcome the shortage of donors for liver transplantation may be the use of hepatocytes for bioartificial devices or transplantation. Therefore, the setting-up of new in vitro culture techniques allowing the long-term survival and functional maintenance of hepatocytes represents a formidable challenge. Aim of this study was to obtain a liver homologous acellular matrix (HAM) able to support viability and metabolic functions of rat hepatocytes in primary culture. HAMs were prepared by sequential incubation of rat liver slices in deoxycholic acid and DNase solutions. Dispersed rat hepatocytes were obtained by collagenase digestion and mechanical disaggregation. Isolated hepatocytes were seeded on uncoated and collagen- or HAM-coated tissue culture plastic wells. Cultures were examined by scanning electron microscopy (SEM), and the viability of hepatocytes and their ability to produce albumin and urea were assessed. The viability of freshly dispersed hepatocytes was about 98%. Hepatocytes seeded on HAM exhibited a significantly higher viability and a markedly lower apoptotic rate than those grown on plastic or collagen. Accordingly, albumin and urea nitrogen productions were significantly higher in HAM-cu...Continue Reading

Citations

Sep 26, 2009·Experimental and Molecular Pathology·Timothy F Dutra, Samuel W French
Mar 30, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Ping ZhouJian Wu
Jul 22, 2014·Journal of Biomedical Materials Research. Part a·Shabnam SabetkishSeyyed Mohammad Tavangar
Jan 18, 2011·Cytotherapy·Francesco Paolo Russo, Maurizio Parola
Aug 31, 2016·Burns and Trauma·Alvin Wen Choong ChuaSi Jack Chong

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