Acer okamotoanum protects SH-SY5Y neuronal cells against hydrogen peroxide-induced oxidative stress

Food Science and Biotechnology
Ji Hyun KimEun Ju Cho

Abstract

Oxidative stress by over-production of reactive oxygen species (ROS) in brain is widely known as a cause of neurodegenerative disease. We investigated protective effects of Acer okamotoanum against oxidative stress by hydrogen peroxide (H2O2) in SH-SY5Y neuronal cells. Acer okamotoanum reduced ROS production and lactate dehydrogenase release in H2O2-induced SH-SY5Y cells, resulting in elevation of cell viability. To elucidate protective mechanisms, we measured inflammation and apoptosis-associated protein expressions. Treatment with A. okamotoanum dose-dependently decreased pro-inflammatory proteins such as inducible nitric oxide synthase and cyclooxygenase-2. Treatment with A. okamotoanum showed down-regulation of pro-apoptosis genes such as cleaved caspase-3, cleaved caspase-9, and Bax, and up-regulation of anti-apoptosis protein including Bcl-2, in H2O2-induced SH-SY5Y cells. We demonstrated potential anti-inflammatory and anti-apoptotic effect of A. okamotoanum in H2O2-induced SH-SY5Y cells. These results suggest that A. okamotoanum may possess neuroprotective potential, but further study is necessary to elucidate its pharmacological effects in neurodegenerative diseases.

References

May 1, 1990·Cytotechnology·A J RacherJ B Griffiths
Aug 1, 1996·Journal of the Neurological Sciences·A M GormanT Cotter
Apr 10, 1997·The New England Journal of Medicine·P J Barnes, M Karin
Apr 29, 1998·The Journal of Biological Chemistry·J G PastorinoJ L Farber
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Sep 30, 2004·Journal of Neuropathology and Experimental Neurology·Luisa Minghetti
Apr 12, 2007·Archives of Pharmacal Research·WenYi JinKiHwan Bae
Feb 18, 2010·International Journal of Molecular Sciences·Chun ShiDavid T Yew
Jun 12, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ningna ZhouJianming Xiang
Oct 9, 2014·BMC Complementary and Alternative Medicine·Alba GarcimartínJuana Benedí
Jul 15, 2015·Environmental Toxicology and Pharmacology·Xiao-Long HuQing-Chun Zhao
May 3, 2016·Journal of Ethnopharmacology·Wu BiPeigen Xiao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis