Acetate acts as a protonophore and differentially affects bead movement and cell migration of the gliding bacterium Cytophaga johnsonae (Flavobacterium johnsoniae).

Microbiology
JoAnn L Dzink-FoxW Godchaux

Abstract

Cells of Cytophaga johnsonae (now Flavobacterium johnsoniae) are able to translocate on solid surfaces but are unable to swim in liquid media. Organelles that may be involved in this gliding motility have not been detected, and the mechanism(s) responsible remains unknown. The movement of latex beads attached to the cell surface is considered by some to be a manifestation of the gliding machinery. In this study, acetate (in nutrient-level quantity, 45 mM) was found to inhibit bead movement on cell surfaces, whilst formation and movement of groups of cells (rafts) and typical colony spread were not affected; generation time (in liquid culture) was only slightly increased. Since acetate is a weak acid and is recognized as a protonophore, various electron-transport-associated features were assessed in an effort to understand the differential effects of acetate on bead movement and cell motility. Selected protonophores and electron transport inhibitors were tested to compare their effects on cell translocation and metabolic activities with those of acetate. Although O2 consumption was not significantly affected in the presence of acetate and the protonmotive force decreased only minimally, ATP levels were markedly decreased. Arsena...Continue Reading

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Citations

Jun 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daisuke NakaneKoji Nakayama
Oct 4, 2005·Journal of Bacteriology·Timothy F BraunMark J McBride
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Mark J McBride
Nov 2, 2007·Journal of Advanced Nursing·Vari DrennanMichael Traynor
Sep 7, 2001·Annual Review of Microbiology·M J McBride
Nov 8, 2017·Journal of Bacteriology·Joseph J JohnstonMark J McBride
Apr 6, 2016·Journal of Bacteriology·Yongtao Zhu, Mark J McBride

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