Acetate metabolism by Escherichia coli in high-cell-density fermentation.

Applied and Environmental Microbiology
G L Kleman, W R Strohl

Abstract

Little is known about the cellular physiology of Escherichia coli at high cell densities (e.g., greater than 50 g [dry cell weight] per liter), particularly in relation to the cellular response to different growth conditions. E. coli W3100 cultures were grown under identical physical and nutritional conditions, by using a computer-controlled fermentation system which maintains the glucose concentration at 0.5 g/liter, to high cell densities at pH values of 6.0, 6.5, 7.0, and 7.5. The data suggest a relationship between the pH of the environment and the amount of acetate excreted by the organism during growth. At pH values of 6.0 and 6.5, the acetate reached a concentration of 6 g/liter, whereas at pH 7.5, the acetate reached a concentration of 12 g/liter. Furthermore, at pH values of 6.0 to 7.0, the E. coli culture undergoes a dramatic metabolic switch in which oxygen and glucose consumption and CO2 evolution all temporarily decreased by 50 to 80%, with a concomitant initiation of acetate utilization. After a 30-min pause in which approximately 50% of the available acetate is consumed, the culture recovers and resumes consuming glucose and oxygen and producing acetate and CO2 at preswitch levels. During the switch period, the s...Continue Reading

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Citations

Jul 14, 2000·Journal of Bacteriology·S KumariA J Wolfe
Oct 1, 2005·Journal of Industrial Microbiology & Biotechnology·Daniel MandersonYusuf Chisti
Apr 29, 2006·Applied Microbiology and Biotechnology·Mark V BaevJohn W Campbell
Aug 2, 2007·Journal of Industrial Microbiology & Biotechnology·Marjan De MeyErick Vandamme
Nov 19, 2008·Applied Microbiology and Biotechnology·Hengwei WangDongzhi Wei
Feb 18, 2016·Bioprocess and Biosystems Engineering·Moritz von StoschRui Oliveira
Jul 17, 2004·Journal of Industrial Microbiology & Biotechnology·Mansi El-Mansi
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Alan J Wolfe
Nov 27, 2002·Applied and Environmental Microbiology·Sriram SrinivasanTillman U Gerngross
Aug 6, 2005·Biotechnology Progress·Cheryl R DittrichKa-Yiu San
Aug 12, 2006·Biotechnology Letters·Geoffrey M SmithElliot Altman
Aug 16, 2014·Applied Microbiology and Biotechnology·Yalun ArifinLars K Nielsen
Dec 20, 2014·Applied Microbiology and Biotechnology·Sara Castaño-CerezoManuel Cánovas
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Jiaming CaoHanjie Ying
Jul 5, 2015·Applied Microbiology and Biotechnology·Janja TrčekLaura R Jarboe
Jul 11, 2014·Antonie van Leeuwenhoek·Pilar Eliana Puentes-Téllez, Jan Dirk van Elsas
Aug 9, 2006·Biotechnology and Bioengineering·M CánovasJ L Iborra
Sep 24, 2019·Journal of Biotechnology·Gabriela Pannunzio Carmignotto, Adriano Rodrigues Azzoni

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