Acetogenesis from dichloromethane by a two-component mixed culture comprising a novel bacterium.

Applied and Environmental Microbiology
A MagliT Leisinger

Abstract

A strictly anaerobic two-component culture able to grow exponentially with a doubling time of 20 h on a medium containing dichloromethane as the carbon and energy source was characterized. On a medium without sulfate, we observed (per mol of dichloromethane) a mass balance of 2 mol of chloride, 0.26 mol of acetate, 0.05 mol of formate, and 0.25 mol of carbon in biomass. One component of the culture, strain DMB, was identified by a 16S ribosomal DNA analysis as a Desulfovibrio sp. The other component, the gram-positive organism strain DMC, could not be isolated. It was possible, however, to associate strain DMC on a medium containing dichloromethane in a coculture with Acetobacterium woodii or Methanospirillum hungatei. Coculture of strain DMC with the Archaeon M. hungatei allowed us to specifically amplify by PCR the 16S rRNA gene of strain DMC. A phylogenetic analysis of the 16S ribosomal DNA sequence revealed that this organism groups within the radiation of the Clostridium-Bacillus subphylum and exhibits the highest levels of sequence similarity (89%) with Desulfotomaculum orientis and Desulfitobacterium dehalogenans. Since the novel organism strain DMC was able to grow acetogenically with dichloromethane when it was associa...Continue Reading

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Citations

Dec 20, 2011·Applied and Environmental Microbiology·Shandra D Justicia-LeonFrank E Löffler
May 9, 1998·Applied and Environmental Microbiology·M E HinesR C Harriss
Nov 29, 2011·Environmental Microbiology·Matthew LeeMike Manefield
May 9, 1998·Applied and Environmental Microbiology·T VannelliT Leisinger
Nov 9, 2006·Biodegradation·Fatih Karadagli, Bruce E Rittmann
Mar 5, 2016·Genome Announcements·Sara KleindienstFrank E Löffler
Dec 14, 2005·Applied and Environmental Microbiology·A MägliT Leisinger
Dec 8, 2017·Frontiers in Microbiology·Justin WrightRegina Lamendella
Jan 20, 2019·Applied and Environmental Microbiology·Sara KleindienstFrank E Löffler
Jun 1, 1996·Current Opinion in Biotechnology·T Leisinger
Jun 1, 1996·Current Opinion in Biotechnology·L P Wackett
Jun 1, 1997·Current Opinion in Biotechnology·G Wohlfarth, G Diekert
Aug 2, 2005·Environmental Science & Technology·Fatih Karadagli, Bruce E Rittmann

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