PMID: 15244053Jul 13, 2004Paper

Acetoin production as an indicator of growth and metabolic inhibition of Listeria monocytogenes

Journal of Applied Microbiology
T L Romick, H P Fleming

Abstract

It has been shown that Listeria monocytogenes produces acetoin from glucose under aerobic conditions. A defined medium with glucose as the sole carbon source was used in an aerobic shake flask culture to reliably produce acetoin. Acetoin, the reactive compound in the Voges-Proskauer test, was assayable in the medium and was used to quantify the metabolic response when inhibitors were added to the medium. Inhibitors such as lactic, acetic, propionic and benzoic acids were used to demonstrate the utility of acetoin production as an indicator of metabolic disruption. With increasing levels of inhibitor, the metabolic and growth responses were measured by acetoin production and optical density change, respectively. Both measurements decreased in a similar manner with increasing inhibitor concentrations. The data also showed the apparent mode of action of the inhibitors. A bacteriostatic effect was observed for the protonated organic acids, acetic (4 mmol l(-1)) and propionic (4 mmol l(-1)), whereas protonated lactic (4 mmol l(-1)) and benzoic (0.16 mmol l(-1)) acids gave an irreversible (apparent bacteriocidal) effect. Lactic, acetic, and propionic acids showed stimulation of metabolic activity at low concentrations, but benzoic di...Continue Reading

Citations

Oct 4, 2006·Infection and Immunity·Sang Sun Yoon, John J Mekalanos
Apr 2, 2013·Canadian Journal of Microbiology·James L SmithGeorge C Paoli
Aug 28, 2007·Microbes and Infection·Biju Joseph, Werner Goebel
Jan 15, 2014·Biotechnology Advances·Zijun Xiao, Jian R Lu
Nov 19, 2011·FEMS Microbiology Letters·Moira MadeoConor P O'Byrne
Jan 20, 2018·International Journal of Molecular Sciences·Ariadna S Sánchez-LópezSofie Thijs
Mar 16, 2017·Archives of Microbiology·Nathan WallaceYvonne Sun
May 10, 2019·Journal of Oral Microbiology·Petra Ganas, Falk Schwendicke
Jul 28, 2020·Biotechnology and Bioengineering·Sowmiya BalasubramanianRamalingam Subramanian
Jun 14, 2019·Frontiers in Microbiology·Valeria ImperatoSofie Thijs
Jun 3, 2021·Pathogens·Bruno Henrique Silva DiasChoong-Min Ryu

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