Acetolysis of Pichia pastoris IFO 0948 strain mannan containing alpha-1,2 and beta-1,2 linkages using acetolysis medium of low sulfuric acid concentration

Archives of Biochemistry and Biophysics
H KobayashiS Suzuki

Abstract

To obtain manno-oligosaccharides containing beta-1,2-linked nonreducing terminal groups from the mannan of Pichia pastoris IFO 0948 strain by acetolysis, an attempt was made to establish the reaction conditions under which cleavage of the alpha-1,6 linkage took place preferentially leaving manno-oligosaccharides composed largely of beta-1,2 linkages. By the action of an ordinary acetolysis medium, a 10/10/1 (v/v) mixture of acetic anhydride, acetic acid, and sulfuric acid at 40 degrees C for 13 h or at 25 degrees C for 120 h, the O-acetyl derivative of this mannan gave mannose, mannobiose, mannotriose, and mannopentaose. However, treatment of the same O-acetyl mannan with a 50/50/1 (v/v) acetolysis medium at 40 degrees C for 15 h gave a mannotetraose in addition to mannose, mannobiose, mannotriose, and mannopentaose. Use of a 100/100/1 (v/v) acetolysis medium at 40 degrees C for 36 h gave a more satisfactory result, a mixture of oligosaccharides, from mannose to mannopentaose, which contained more mannotetraose than mannopentaose. Because both mannotetraose and mannopentaose contained alpha-1,2 and beta-1,2 linkages, it was concluded that an acetolysis medium containing a low concentration of sulfuric acid, up to 0.5% (v/v), fa...Continue Reading

References

Dec 1, 1969·Journal of Bacteriology·J Kocourek, C E Ballou
May 1, 1968·Biochemistry·T S Stewart, C E Ballou
Jan 1, 1984·Microbiology and Immunology·M FunayamaY Fukazawa

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Citations

Aug 15, 1988·Carbohydrate Research·E KatzenellenbogenE Romanowska
Oct 10, 2006·Applied and Environmental Microbiology·Paul J WeimerWillem H Van Zyl
Oct 27, 2004·Infection and Immunity·James Masuoka, Kevin C Hazen
Jun 26, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Nobuyuki ShibataShigeo Suzuki
Oct 7, 1997·FEBS Letters·K IkutaY Okawa
Jan 8, 1999·Biochimica Et Biophysica Acta·T R Gemmill, R B Trimble
Oct 1, 1994·Infection and Immunity·H KobayashiS Suzuki
Nov 27, 2004·Journal of Molecular Recognition : JMR·Rachel Daly, Milton T W Hearn
Apr 3, 2020·ACS Infectious Diseases·Lufeng YanRobert J Linhardt

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