Acetonic extracts of the endolichenic fungus EL002332 isolated from Endocarpon pusillum exhibits anticancer activity in human gastric cancer cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Yi YangHangun Kim

Abstract

Endolichenic fungi are microbes that inhabit the thalli of lichens and produce various unique chemicals that can be used for pharmaceutical purposes. This study screened a library of endolichenic fungal extracts to identify novel anticancer agents capable of suppressing the tumorigenicity of human cancer cells. Active compounds were isolated from extracts of endolichenic fungi by column chromatography and reverse-phase HPLC. The anticancer effects of the extracts on cell viability was assessed with the use of MTT assay, Western blotting, fluorescence labeling of apoptotic cell, and flow cytometric analysis; and cell motility with the use of migration, invasion and soft agar colony-formation assay in vitro; and on skin and intraperitoneal mouse xenograft tumors in vivo were investigated. The therapeutic effects of the extract alone or in combination with the conventional chemoreagent docetaxel were analyzed by sulforhodamine B assay. Acetone extracts of EL002332, isolated from Endocarpon pusillum collected in the China desert in 2010, showed selective cytotoxicity against AGS human gastric cancer cells and CT26 mouse colon cancer cells. An active pure compound named myC was isolated from mycelium acetone extracts in a liquid cul...Continue Reading

Citations

Jan 18, 2020·Biomolecules·Zuzana SolárováPeter Solár
Mar 11, 2020·Expert Opinion on Drug Discovery·Mahshid MohammadiLew Christopher
Nov 18, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi YangHangun Kim
Mar 27, 2020·Gastroenterology Research and Practice·Wenjie LiHong Zhu
May 31, 2021·Seminars in Cancer Biology·Tanvir Ul Hassan DarShafiul Haque
Aug 2, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·İsa TaşHangun Kim

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