Acetoxy-acetylaminofluorene-modified dGTP can be used to label oligonucleotides or DNA enzymatically

Molecular and Cellular Probes
J AuriolJ L Guesdon

Abstract

A competitive enzyme immunoassay using a bispecific monoclonal antibody (Bi-MAb) was developed to quantify acetylaminofluorene (AAF) adducts fixed on DNA and then compared to the spectrophotometric method. It was shown that this simple method allowed the measurement of as low as 2 pmol per assay of AAF bound to DNA. This technique was used to monitor synthesis and purification of N-acetoxy-N-2-acetylaminofluorene modified dGTP (AAF-dGTP). It was shown that AAF-dGTP can be a substrate for the terminal deoxynucleotidyl transferase. Finally, using the Bi-MAb we developed a non-radioactive sandwich hybridization assay making use of oligonucleotide covalently bound to microwells and of synthetic oligonucleotide tailed with AAF-dGTP as a probe.

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Jan 11, 1990·Nucleic Acids Research·D ThierryJ L Guesdon
Jun 1, 1987·Molecular and Cellular Probes·P DahlénT Lövgren
May 11, 1988·Nucleic Acids Research·A F CookC L Brakel
Aug 26, 1985·Nucleic Acids Research·D G Sanford, T R Krugh
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P TchenM Leng
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M F DuboisP Tiollais

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