Acetyl-11-Keto-β-Boswellic Acid Exerts the Anti-Cancer Effects via Cell Cycle Arrest, Apoptosis Induction and Autophagy Suppression in Non-Small Cell Lung Cancer Cells

OncoTargets and Therapy
Minghe LvTiankui Qiao

Abstract

Acetyl-11-keto-β-boswellic acid (AKBA) is a triterpenoid, which is the main component of boswellic acid from Boswellia Serrata, a medicinal plant that has shown immense potential in anti-cancer therapy. This study aims to explore the roles and molecular mechanisms of AKBA on cell behavior in non-small cell lung cancer (NSCLC) cells. The effects of AKBA on the cell viability in A549, H460, H1299, and BEAS-2B cells were determined by the CCK-8 assay. The colony formation assay was used to identify the effects of AKBA on cell proliferation. Potential roles of AKBA in regulating the cell cycle, apoptosis, and autophagy in A549 were evaluated by flow cytometry, Western blotting, reverse transcription-polymerase chain reaction (PCR) and immunofluorescence (IF). AKBA reduced cell viability in A549, H460, H1299, and BEAS-2B. In A549 cells, AKBA suppressed the clone formation, arrested the cell cycle at the G0/G1 phase, induced cellular apoptosis. We found that AKBA suppressed the formation of autolysosome, and decreased the expression levels of Beclin-1, LC3A/B-I, and LC3A/B-II proteins. Furthermore, AKBA also inhibited the expression levels of PI3K/Akt signaling pathway proteins. AKBA exerts the anti-cancer effects via cell cycle arre...Continue Reading

Citations

Feb 2, 2021·Phytochemistry·Ahmed Al-HarrasiRené Csuk
Nov 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wamidh H TalibAsma Ismail Mahmod
Apr 18, 2021·The Journal of Pharmacy and Pharmacology·Bo CaoLi Han
May 8, 2021·Journal of Pharmaceutical and Biomedical Analysis·Michael Zwerger, Markus Ganzera

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Methods Mentioned

BETA
Flow Cytometric
flow cytometry
protein assay
PCR

Software Mentioned

GraphPad Prism
GraphPad Excel

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