Acetyl Coenzyme A Acetyltransferase of Rhizobium sp. (Cicer) Strain CC 1192.

Applied and Environmental Microbiology
S A Kim, Les Copeland

Abstract

To investigate why Rhizobium sp. (Cicer) strain CC 1192 cells accumulate poly-R-3-hydroxybutyrate in the free-living state but not as bacteroids in nodules on chickpea (Cicer arietinum L.) plants, we have examined the kinetic properties of acetyl coenzyme A (acetyl-CoA) acetyltransferase (also known as acetoacetyl-CoA thiolase and 3-ketothiolase [EC 2.3.1.9]) from both types of cells. The enzyme had a native molecular mass of 180 (plusmn) 4 kDa, and the subunit molecular mass was 44 (plusmn) 1 kDa. The seven amino acids from the N terminus were Lys-Ala-Ser-Ile-Val-Ile-Ala. Thiolysis and condensation activity of the enzyme from free-living CC 1192 cells were optimal at pHs 7.8 and 8.1, respectively. The relationship between substrate concentrations and initial velocity for the thiolysis reaction were hyperbolic and gave K(infm) values for acetoacetyl-CoA and CoA of 42 and 56 (mu)M, respectively. The maximum velocity in the condensation direction was approximately 10% of that of the thiolysis reaction. With highly purified preparations of the enzyme, a value of approximately 1 mM was determined for the apparent K(infm) for acetyl-CoA. However, with partially purified enzyme preparations or when N-ethylmaleimide was included in re...Continue Reading

References

Sep 1, 1976·Archives of Biochemistry and Biophysics·G R Duncombe, F E Frerman
Jan 23, 1978·Archives of Microbiology·T NishimuraK Tomita
Mar 12, 1975·Archives of Microbiology·H Berndt, H G Schlegel
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May 1, 1973·The Biochemical Journal·P J Senior, E A Dawes
Jan 1, 1981·Methods in Enzymology·H Schulz, H Staack
Nov 1, 1996·Applied and Environmental Microbiology·S A Kim, L Copeland

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Citations

Feb 5, 2008·Biotechnology Letters·Jason J Reddick, Jayme K Williams
Aug 4, 1998·Applied and Environmental Microbiology·S N Chohan, L Copeland
Jun 4, 2019·Metabolites·Diana Montes-GrajalesEsperanza Martinez-Romero

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