Acetylated chitosan oligosaccharides act as antagonists against glutamate-induced PC12 cell death via Bcl-2/Bax signal pathway

Marine Drugs
Cui HaoChunxia Li

Abstract

Chitosan oligosaccharides (COSs), depolymerized products of chitosan composed of β-(1→4) D-glucosamine units, have broad range of biological activities such as antitumour, antifungal, and antioxidant activities. In this study, peracetylated chitosan oligosaccharides (PACOs) and N-acetylated chitosan oligosaccharides (NACOs) were prepared from the COSs by chemcal modification. The structures of these monomers were identified using NMR and ESI-MS spectra. Their antagonist effects against glutamate-induced PC12 cell death were investigated. The results showed that pretreatment of PC12 cells with the PACOs markedly inhibited glutamate-induced cell death in a concentration-dependent manner. The PACOs were better glutamate antagonists compared to the COSs and the NACOs, suggesting the peracetylation is essential for the neuroprotective effects of chitosan oligosaccharides. In addition, the PACOs pretreatment significantly reduced lactate dehydrogenase release and reactive oxygen species production. It also attenuated the loss of mitochondrial membrane potential. Further studies indicated that the PACOs inhibited glutamate-induced cell death by preventing apoptosis through depressing the elevation of Bax/Bcl-2 ratio and caspase-3 acti...Continue Reading

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Citations

Jan 31, 2017·Experimental Biology and Medicine·Jin QinYang Liu
Feb 14, 2019·Connective Tissue Research·Ra Jeong KimHyung Bin Park
Jul 29, 2015·World Journal of Gastroenterology : WJG·Hai-Bo ZhouZhi-Gang Chen
May 31, 2017·International Forum of Allergy & Rhinology·Ah-Yeoun JungYoung Hyo Kim
Nov 30, 2019·BioMed Research International·Ilias MarmouziShanaz O Mohamed
Apr 29, 2019·Cellular and Molecular Life Sciences : CMLS·Behrouz FarhadihosseinabadiMazaher Gholipourmalekabadi
Mar 30, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xingyan JuXiaoran Liu

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Methods Mentioned

BETA
acetylation
ELISA
NMR
confocal microscopy

Software Mentioned

Image J
SPSS

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